...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Folia histochemica et cytobiologica >Possible involvement of microtubules and microfilaments of the epididymal epithelial cells in 17beta-estradiol synthesis.
【24h】

Possible involvement of microtubules and microfilaments of the epididymal epithelial cells in 17beta-estradiol synthesis.

机译：附睾上皮细胞的微管和微丝可能参与17β-雌二醇合成。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The rat epididymal epithelial cells revealed features of steroidogenic cells and released 17beta-estradiol (E2) into the culture medium. In steroidogenic cells, elements of the cytoskeleton due to their influence on organelle distribution are implicated in the regulation of steroidogenesis. In the present study, the morphology of cultured epididymal epithelial cells in light, scanning and transmission electron microscopes was evaluated. The organization of microtubules and microfilaments revealed by fluorescence microscopy, and the concentration of E2 in cultured medium were also studied. The epididymal epithelial cells were cultured in different conditions: in the medium with or without exogenous testosterone (T) and in the co-culture with Leydig cells as a source of androgens. The cells in co-culture located close to Leydig cells were rich in glycogen, PAS-positive substances and lipid droplets, in higher amount than the cells cultured with addition of exogenous testosterone. Stress fibers and microtubules of epididymal epithelial cells cultured with exogenous T and in co-culture with Leydig cells presented typical structure, and numerous granular protrusions appeared on the surface of the cells. Disorganization of microtubules and shortening of stress fibers as well as the smooth cell surface deprived of granular protrusions were observed in the epididymal epithelial cells cultured without T. Change of the cytoskeleton organization caused by the absence of androgen in culture medium resulted in an increased E2 secretion.
机译：大鼠附睾上皮细胞显示类固醇生成细胞的特征，并将17β-雌二醇（E2）释放到培养基中。在类固醇生成细胞中，由于其对细胞器分布的影响而导致的细胞骨架成分与类固醇生成的调控有关。在本研究中，在光，扫描和透射电子显微镜中评估了培养的附睾上皮细胞的形态。还研究了荧光显微镜显示的微管和微丝的组织，以及培养基中E2的浓度。附睾上皮细胞在不同条件下培养：在有或没有外源睾酮（T）的培养基中以及与Leydig细胞作为雄激素来源的共培养中。与Leydig细胞靠近的共培养细胞富含糖原，PAS阳性物质和脂质滴，其含量高于添加外源睾丸激素培养的细胞。与外源T培养并与Leydig细胞共培养的附睾上皮细胞的应力纤维和微管呈现出典型的结构，并且在细胞表面上出现了许多颗粒状突起。在没有T的附睾上皮细胞中观察到微管的混乱和应力纤维的缩短以及光滑的细胞表面没有颗粒状突起。由于培养基中缺乏雄激素而引起的细胞骨架组织的变化导致E2分泌增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号