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Embryonic Development of Siberian Sturgeon Acipenser baerii under Hatchery Conditions: An Image Guide with Embryological Descriptions

机译:孵化场条件下西伯利亚urge鱼Acipenser baerii的胚胎发育:带有胚胎学说明的图像指南

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Normal embryonic development at a constant temperature ( ) has been described for the Siberian sturgeon Acipenser baerii (Acipenseriformes). Hormone-induced spawning and artificial insemination were performed to prepare embryonic batches for embryologic examination. After insemination, early cleavages of the Siberian sturgeon embryos continued for 7 h post-fertilization (HPF), showing the typical pattern of uneven holoblastic cleavage. Blastulation and gastrulation began at 9 HPF and 19 HPF, respectively. Epiboly formation (2/3 covered) was observed at 25 HPF during gastrulation. Neurulation was initiated with the formation of a slit-like neural groove from the blastopore at 33 HPF. During neurulation, the primary embryonic kidney (pronephros) and s-shaped heart developed. The embryos underwent progressive differentiation, which is typical of Acipenseriform species. A mass hatching was observed at 130 HPF, and the average total length of the hatched prolarvae was 10.5 mm. The hatched prolarvae possessed a typical pigment plug (yolk plug). The results of this study are valuable not only as a reference guide for the artificial propagation of Siberian sturgeon in hatcheries but also as the basis for the derivation of developmental gene expression assays for this species.
机译:西伯利亚st(Acipenser baerii)(Acipenseriformes)已描述了在恒定温度()的正常胚胎发育。进行激素诱导的产卵和人工授精,以制备用于胚胎学检查的胚胎批次。授精后,西伯利亚st鱼胚胎的早期卵裂在受精后持续7 h(HPF),显示出不均匀的整体裂卵模式。爆破和气化分别从9 HPF和19 HPF开始。在气化过程中,在25 HPF下观察到外延形成(2/3覆盖)。通过在33 HPF下从芽孢形成狭缝状神经沟来开始中和。在培养过程中,发育了主要的胚胎肾脏(前肾)和S形心脏。胚胎经历渐进分化,这是Acipenseriform物种的典型特征。在130 HPF观察到大量孵化,孵出的幼体的平均总长度为10.5 mm。孵化的幼体具有典型的色素塞(蛋黄塞)。这项研究的结果不仅可作为西伯利亚st在孵化场中人工繁殖的参考指南,而且可作为衍生该物种的发育基因表达分析的基础。

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