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首页> 外文期刊>Fisheries and Aquatic Sciences >Transcriptional Alteration of Two Metallothionein Isoforms in Mud Loach (Misgurnus mizolepis) Fry during Acute Heavy Metal Exposure
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Transcriptional Alteration of Two Metallothionein Isoforms in Mud Loach (Misgurnus mizolepis) Fry during Acute Heavy Metal Exposure

机译：急性重金属暴露期间泥Lo（Misgurnus mizolepis）油炸物中两种金属硫蛋白同工型的转录变化

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摘要

Altered mRNA expression of two metallothionein isoforms (MT-IA and MT-IB) in response to acute heavy metal exposure was examined in mud loach, Misgurnus mizolepis, fry using a real-time RTPCR assay. Sublethal exposure (1 or 5 ) to Cd, Cr, Fe, Mn, Ni, and Zn resulted in highly variable transcriptional responses of the two MT isoforms to the heavy metal ions, including upregulation, a steady state, and downregulation. Overall, the most potent inducer of both MT isoforms was Cd (up to 6-fold). Another exposure experiment using a series of doses of Cu revealed that the stimulation patterns of the two MT isoforms differed: MT-IA transcription was soon saturated at higher concentrations (about 2-fold at 1-4 of Cu), whereas the activation of MT-IB was more dependent on the treatment dose (increased up to 5-fold at 3 ). The isoform-specific allotment of constitutive and inducible functions was not as clear in fry as in adult tissues. Coordinated interaction between the MT-IA and MT-IB isoforms was hypothesized based on the finding that MT-IA represented a primary action under 'less stressful' or 'sublethal' conditions, whereas the activation of MT-IB became important under 'more stressful' or 'lethal' circumstances in this species.
机译：使用实时RTPCR测定法，在泥loMisgurnus mizolepis鱼苗中检查了两种金属硫蛋白同工型（MT-IA和MT-IB）响应于急性重金属暴露而改变的mRNA表达。亚致死性（1或5）暴露于Cd，Cr，Fe，Mn，Ni和Zn导致两种MT同工型对重金属离子的转录反应高度可变，包括上调，稳态和下调。总体而言，两种MT同工型的最强诱导剂均为Cd（最高6倍）。另一个使用一系列铜剂量的暴露实验表明，两种MT同工型的刺激模式不同：MT-IA转录在较高浓度下很快饱和（在1-4 Cu时约为2倍），而MT的激活-IB更依赖于治疗剂量（在3处增加至5倍）。组成和诱导功能的同工型特异性分配在鱼苗中不如在成年组织中清楚。根据以下发现假设了MT-IA和MT-IB同工型之间的协同相互作用：在“压力较小”或“亚致死”条件下MT-IA代表主要作用，而在“压力较大”下MT-IB的激活变得重要该物种的“致命”情况。

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