Development of Clamping Probe for Rare DNA Detection using Universal Primers

Kim Meyong Il; Lee Ki-Young; Cho Sang-Man

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Development of Clamping Probe for Rare DNA Detection using Universal Primers

机译：通用引物用于稀有DNA检测的钳位探针的开发

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PCR amplification with universal primer is a useful tool for speciation of symbionts in marine eukaryote coupled with robust separation method such as denaturing high performance chromatography (DHPLC). To overcome the biased amplification, clamping PCR is recommended to suppress the amplification of host gene. In this study, we evaluated the efficiency of rare gene detection for two kinds of clamping probes which were successfully utilized for eukaryotic symbiont analysis: C3 linked nucleotide (C3) and peptide nucleic acid (PNA). PNA was 3-4 orders of magnitude higher than that of C3 tested in clamping efficiency and rare gene detection. This represented that PNA could be a more competent clamping probe for the enhancement of PCR amplification for rare symbiont genes.

机译：通用引物的PCR扩增是海洋真核生物中共生体形成的有用工具，结合了稳健的分离方法（如变性高效色谱法（DHPLC））。为了克服偏向扩增，建议使用钳制PCR抑制宿主基因的扩增。在这项研究中，我们评估了成功用于真核生物共生体分析的两种夹紧探针的稀有基因检测效率：C3连接核苷酸（C3）和肽核酸（PNA）。在钳位效率和稀有基因检测方面，PNA比C3高3-4个数量级。这表明PNA可能是一种更有效的钳位探针，可用于增强稀有共生体基因的PCR扩增。

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《Fisheries and Aquatic Sciences》 |2014年第3期|共6页
作者
Kim Meyong Il; Lee Ki-Young; Cho Sang-Man;
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1. Development of Clamping Probe for Rare DNA Detection using Universal Primers [J] . Meyong il Kim, Ki-Young Lee, Sang-Man Cho Fisheries and Aquatic Sciences . 2014,第3期

机译：通用引物用于稀有DNA检测的钳位探针的开发
2. Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe [J] . Luo JD, Chan EC, Shih CL, Nucleic Acids Research . 2006,第2期

机译：使用肽核酸作为PCR钳和传感器探针，在单管反应中检测稀有突变K-ras DNA
3. Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe [J] . Ji-Dung Luo, Err-Cheng Chan, Chun-Liang Shih, Nucleic Acids Research . 2006,第2期

机译：使用肽核酸作为PCR钳和传感器探针，在单管反应中检测稀有突变K-ras DNA
4. Identification of urinary pathogens with species-specific 16s rDNA probes under rapid detection time at ambient temperature for the development of a MEMsbased urinary pathogen DNA biosensor [C] . Chien-Pin Sun, Joseph C. Liao, Yao-Hua Zhang, The 2nd World Congress for Chinese Biomedical Engineers . 2004

机译：在室温下快速检测时间下，用物种特异性的16s rDNA探针鉴定尿中病原体，以开发基于MEMs的尿中病原体DNA生物传感器
5. Mechanism of electron transfer in double-stranded DNA and PNA-DNA hybrids, and the development of a fluorescence probe for DNA and RNA detection. [D] . Ly, Danith. 1998

机译：电子在双链DNA和PNA-DNA杂化物中转移的机理，以及用于DNA和RNA检测的荧光探针的发展。
6. Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe [O] . Ji-Dung Luo, Err-Cheng Chan, Chun-Liang Shih, 2006

机译：使用肽核酸作为PCR钳和传感器探针在单管反应中检测稀有突变K-ras DNA
7. Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe [O] . Luo, Ji-Dung, Chan, Err-Cheng, Shih, Chun-Liang, 2006

机译：使用肽核酸作为PCR钳和传感器探针，在单管反应中检测稀有突变K-ras DNA

Development of Clamping Probe for Rare DNA Detection using Universal Primers

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