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首页> 外文期刊>Fisheries and Aquatic Sciences >Comparison of the Exopeptidase Activity of Fractions from Crude Extracts of Octopus Octopus vulgaris Cuvier Hepatopancreas Using Different Fractionation Methods
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Comparison of the Exopeptidase Activity of Fractions from Crude Extracts of Octopus Octopus vulgaris Cuvier Hepatopancreas Using Different Fractionation Methods

机译：八达通章鱼粗制肝胰脏粗提物中不同组分分离方法的肽外切酶活性比较

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This study was performed to identify the optimum fractionation method and conditions to obtain exopeptidase-active fractions from octopus hepatopancreas (HP) crude extracts (CEs) using four techniques: solid ammonium sulfate fractionation, polyethylene glycol (PEG) fractionation, anion exchange chromatography, and gel filtration chromatography. The fractions with the highest total activity toward L-leucine-p-nitroanilide (Leu-pNA) were fraction IV from the ammonium sulfate and PEG fractionation, and fraction II in ion exchange and gel filtration chromatography. The total exoprotease activity of these fractions was highest in fraction IV (4,050.20 U) of ammonium sulfate fractionation, followed by fraction II (3,600.28 U) from gel filtration chromatography, fraction IV (2,861.30 U) from PEG fractionation, and fraction II (2,576.28 U) from ion exchange chromatography. These results suggest that ammonium sulfate fractionation using 60-80% ammonium sulfate was the most efficient method for separating the exoprotease active fractions from CEs of octopus HP.
机译：这项研究旨在确定最佳的分馏方法和条件，以使用四种技术从章鱼肝胰腺（HP）粗提取物（CEs）中获得肽外肽酶活性馏分：固态硫酸铵分馏，聚乙二醇（PEG）分馏，阴离子交换色谱和凝胶过滤色谱。对L-亮氨酸-对硝基苯胺（Leu-pNA）具有最高总活性的馏分是硫酸铵和PEG馏分的馏分IV，以及离子交换和凝胶过滤色谱法的馏分II。这些级分的总外切蛋白酶活性在硫酸铵级分的级分IV（4,050.20 U）中最高，其次是凝胶过滤色谱法的级分II（3,600.28 U），PEG级分的级分IV（2,861.30 U）和级分II（2,576.28 U） ）。这些结果表明，使用60-80％硫酸铵分级分离硫酸铵是从章鱼HP CE分离外切蛋白酶活性组分的最有效方法。

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