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首页> 外文期刊>Fisheries and Aquatic Sciences >Initial Culture Conditions for Primary Cell Populations Derived from Radula Tissue in Abalone Haliotis discus hannai
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Initial Culture Conditions for Primary Cell Populations Derived from Radula Tissue in Abalone Haliotis discus hannai

机译：鲍鱼鲍氏鲍鱼（Haliotis discus hannai）Rad管组织衍生的原代细胞群体的初始培养条件

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Abalone immortal cell lines can be used to study the physiological properties and disease mechanisms of abalone at the cellular and molecular level. As a first step for the final goal to establish abalone immortal cell lines, we examined various initial culture conditions for primary cell populations derived from Haliotis discus hannai radula tissue. The survival rate after cell isolation procedures using the enzymatic method was as low as . Based on three different experimental conditions for H. discus hannai radula-derived cell culture, we found that the salinity of the media and the presence of growth-promoting factors were important to support radula-derived primary cell populations during the initial culture. The growth factor-containing media adjusted to 35 psu salinity could induce 100% (8 out of 8 trials) initial cell attachment, and the rate of cell attachment reached 50-70%. The data obtained from this study will provide useful information for developing immortal cell lines from abalone species.
机译：鲍鱼永生细胞系可用于在细胞和分子水平研究鲍鱼的生理特性和疾病机理。作为建立鲍鱼永生细胞系的最终目标的第一步，我们研究了源自Halotis discus hannai radula组织的原代细胞群体的各种初始培养条件。使用酶法分离细胞后的存活率低至。基于三种不同的条件，汉逊铁心的胚培养细胞，我们发现培养基的盐度和生长促进因子的存在对于在初始培养过程中支持的原代细胞群很重要。调整至盐度为35 psu的含生长因子的培养基可诱导100％（8个试验中的8个）初始细胞附着，细胞附着率达到50-70％。从这项研究中获得的数据将为从鲍鱼物种发展永生细胞系提供有用的信息。

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