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首页> 外文期刊>Fisheries science >Paralytic shellfish poisoning toxin analysis of the genus Alexandrium (Dinophyceae) occurring in Korean coastal waters
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Paralytic shellfish poisoning toxin analysis of the genus Alexandrium (Dinophyceae) occurring in Korean coastal waters

机译：韩国沿海水域亚历山大藻（Dinophyceae）的麻痹性贝类中毒毒素分析

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Paralytic shellfish poisoning (PSP) toxins produced by Alexandrium isolates from Korea were analyzed by high-pressure liquid chromography. Species designation of the regional isolates was determined by morphological criteria and ribotyping inferred from sequences of the 28S rDNA D1–D2 region. Toxin analysis performed at the exponential growth phase, revealed that the two strains of A. fraterculus were non-toxic, while the strains of A. tamarense and A. catenella were toxic. Toxic isolates DPC7 and DPC8 of A. catenella produced GTX1, 2, 3, 4, 5, dcGTX2, 3, C1, 2, neo STX and STX with trace or non-detectable levels of C3 and C4, while isolates UL7, KDW981, SJW97043, SJW97046, KJC97111 and KJC97112 of A. tamarense produced GTX1, 2, 3, 4, dcGTX3, C1, 2, neoSTX with trace or non-detectable levels of C3, 4, dcSTX and STX, and no GTX5 and dcGTX2. The major toxins produced by A. catenella were C1+2, and those of A. tamarense were C1+2 and GTX4 in most of the isolates. A tamarense strains other than SJW97046 produced a relatively high proportion of carbamate toxins, reflecting the high toxicity scores of shellfish intoxication in sampled coastal areas. Two representative toxic isolates, A. tamarense SJW97043 and A. catenella DPC7, were cultured for 30 days in batch mode and subjected to toxin analysis at 5-day intervals. Comparison of toxin productivity in terms of total toxin content, toxin components, and their variations with culture age revealed marked differences between the two strains.
机译：用高压液相色谱法分析了韩国亚历山大藻分离株产生的麻痹性贝类中毒（PSP）毒素。通过形态学标准和从28S rDNA D1-D2区序列推断的核糖型确定区域分离株的种类。在指数生长期进行的毒素分析表明，两个小曲霉菌株均无毒，而塔玛生曲霉和链状芽孢杆菌均具有毒性。链球菌的有毒分离物DPC7和DPC8产生了GTX1、2、3、4、5，dcGTX2、3，C1、2，neo STX和STX以及痕量或不可检测的C3和C4水平，而分离物UL7，KDW981，番茄的SJW97043，SJW97046，KJC97111和KJC97112生产的GTX1、2、3、4，dcGTX3，C1、2，neoSTX具有痕量或不可检测的C3、4，dcSTX和STX水平，而没有GTX5和dcGTX2。在大多数分离物中，链状假单胞菌产生的主要毒素是C1 + 2，而他玛曲霉产生的主要毒素是C1 + 2和GTX4。除SJW97046以外的其他tamarense菌株产生了相对较高比例的氨基甲酸酯毒素，这反映了在采样的沿海地区贝类中毒的毒性评分很高。将两种代表性的有毒分离株，番茄红曲霉SJW97043和链状芽孢杆菌DPC7，以分批模式培养30天，并每隔5天进行毒素分析。在总毒素含量，毒素成分及其随培养年龄的变化方面比较了毒素生产率，发现这两种菌株之间存在显着差异。

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