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首页> 外文期刊>Frontiers in Microbiology >Resistance Mutations in gyrA and parC are Common in Escherichia Communities of both Fluoroquinolone-Polluted and Uncontaminated Aquatic Environments
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Resistance Mutations in gyrA and parC are Common in Escherichia Communities of both Fluoroquinolone-Polluted and Uncontaminated Aquatic Environments

机译：在 Escherichia 社区中，被氟喹诺酮污染和未污染的水生环境中常见的 gyrA 和 parC 突变很常见。

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Alterations in the target proteins of fluoroquinolones, especially in GyrA and ParC, are known to cause resistance. Here, we investigated environmental Escherichia communities to explore the possible link between the abundance of mutations, and the exposure to fluoroquinolones. Sediment samples were collected from a relatively pristine lake, up and downstream from a sewage treatment plant, and from several industrially polluted sites. The quinolone resistance-determining regions of gyrA and parC were analyzed using amplicon sequencing of metagenomic DNA. Five non-synonymous substitutions were present in all samples, and all of these mutations have been previously linked to fluoroquinolone resistance in Escherichia coli . In GyrA, substitutions S83L and D87N were on average detected at frequencies of 86 and 32%, respectively, and 31% of all amplicons encoded both substitutions. In ParC, substitutions S80I, E84G, and E84V were detected in 42, 0.9, and 6.0% of the amplicons, respectively, and 6.5% encoded double substitutions. There was no significant correlation between the level of fluoroquinolone pollution and the relative abundance of resistance mutations, with the exception of the most polluted site, which showed the highest abundance of said substitutions in both genes. Our results demonstrate that resistance mutations can be common in environmental Escherichia , even in the absence of a fluoroquinolone selective pressure.
机译：已知氟喹诺酮类药物的靶蛋白发生变化，尤其是GyrA和ParC中的变化，会引起耐药性。在这里，我们调查了环境大肠埃希氏菌群落，以探讨大量突变与氟喹诺酮类药物暴露之间的可能联系。从相对原始的湖泊，污水处理厂的上游和下游以及几个工业污染地点收集沉积物样本。使用宏基因组DNA的扩增子测序分析了gyrA和parC的喹诺酮耐药性决定区域。在所有样品中存在五个非同义取代，并且所有这些突变先前都与大肠杆菌中的氟喹诺酮耐药性有关。在GyrA中，平均分别在86和32％的频率处检测到置换S83L和D87N，并且所有扩增子中有31％编码了这两个置换。在ParC中，分别在42％，0.9％和6.0％的扩增子中检测到S80I，E84G和E84V取代，而6.5％的编码为双取代。氟喹诺酮污染水平和耐药突变的相对丰度之间没有显着相关性，除了污染最严重的位点外，这两个基因中所述取代的丰度最高。我们的结果表明，即使在没有氟喹诺酮选择压力的情况下，抗性突变在环境大肠杆菌中也很常见。

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