...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Microbiology >Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes
【24h】

Analysis of methane-producing and metabolizing archaeal and bacterial communities in sediments of the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes

机译：通过 mcrA 和 pmoA 基因的PCR扩增揭示了南海北部和米埔海岸自然保护区沉积物中甲烷产生和代谢的古细菌和细菌群落

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Communities of methanogens, anaerobic methanotrophic archaea and aerobic methanotrophic bacteria (MOB) were compared by profiling polymerase chain reaction (PCR)-amplified products of mcrA and pmoA genes encoded by methyl-coenzyme M reductase alpha subunit and particulate methane monooxygenase alpha subunit, respectively, in sediments of northern South China Sea (nSCS) and Mai Po mangrove wetland. Community structures representing by mcrA gene based on 12 clone libraries from nSCS showed separate clusters indicating niche specificity, while, Methanomicrobiales, Methanosarcinales clades 1,2, and Methanomassiliicoccus -like groups of methanogens were the most abundant groups in nSCS sediment samples. Novel clusters specific to the SCS were identified and the phylogeny of mcrA gene-harboring archaea was updated. Quantitative polymerase chain reaction was used to detect mcrA gene abundance in all samples: similar abundance of mcrA gene in the surface layers of mangrove (3.4～3.9 × 10~(6)copies per gram dry weight) and of intertidal mudflat (5.5～5.8 × 10~(6)copies per gram dry weight) was observed, but higher abundance (6.9 × 10~(6)to 1.02 × 10~(8)copies per gram dry weight) was found in subsurface samples of both sediment types. Aerobic MOB were more abundant in surface layers (6.7～11.1 × 10~(5)copies per gram dry weight) than the subsurface layers (1.2～5.9 × 10~(5)copies per gram dry weight) based on pmoA gene. Mangrove surface layers harbored more abundant pmoA gene than intertidal mudflat, but less pmoA genes in the subsurface layers. Meanwhile, it is also noted that in surface layers of all samples, more pmoA gene copies were detected than the subsurface layers. Reedbed rhizosphere exhibited the highest gene abundance of mcrA gene (8.51 × 10~(8)copies per gram dry weight) and pmoA gene (1.56 × 10~(7)copies per gram dry weight). This study investigated the prokaryotic communities responsible for methane cycling in both marine and coastal wetland ecosystems, showing the distribution characteristics of mcrA gene-harboring communities in nSCS and stratification of mcrA and pmoA gene diversity and abundance in the Mai Po Nature Reserve.
机译：比较了产甲烷菌，厌氧甲烷营养古细菌和需氧甲烷营养细菌（MOB）的组成，方法分别为由甲基辅酶M还原酶α亚基和颗粒甲烷单加氧酶α亚基编码的聚合酶链反应（PCR）扩增的mcrA和pmoA基因产物，位于南海北部和米埔红树林湿地的沉积物中。基于来自nSCS的12个克隆文库的mcrA基因所代表的群落结构显示出独立的簇，表明具有生态位特异性，而甲烷菌微团，甲烷单胞菌属进化枝1,2和产甲烷球菌样甲烷菌群是nSCS沉积物样品中最丰富的组。鉴定了新的集群特有的SCS，并更新了系mcrA基因的古细菌的系统发育。定量聚合酶链反应用于检测所有样品中的mcrA基因丰度：在红树林表层（每克干重3.4〜3.9×10〜（6）份）和潮间带滩涂（5.5〜5.8）中类似的mcrA基因丰度。每克干重×10〜（6）份，但在两种沉积物类型的地下样品中均发现较高的丰度（每克干重6.9×10〜（6）至1.02×10〜（8）份）。基于pmoA基因，好氧MOB的表层（每克干重6.7〜11.1×10〜（5）份）比地下层（每克干重1.2〜5.9×10〜（5）份）丰富。与潮间带滩涂相比，红树林表层具有更多的pmoA基因，而在表层以下的pmoA基因较少。同时，还注意到在所有样品的表层中，检测到的pmoA基因拷贝比表层下更多。芦苇根际的mcrA基因丰度最高（每克干重8.51×10〜（8）份）和pmoA基因最高（每克干重1.56×10〜（7）份）。这项研究调查了海洋和沿海湿地生态系统中负责甲烷循环的原核生物群落，显示了nSCS中携带mcrA基因的群落的分布特征以及米埔自然保护区中mcrA和pmoA基因多样性和丰度的分层。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号