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首页> 外文期刊>Frontiers in Microbiology >Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines
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Streptomyces colonosanans sp. nov., A Novel Actinobacterium Isolated from Malaysia Mangrove Soil Exhibiting Antioxidative Activity and Cytotoxic Potential against Human Colon Cancer Cell Lines

机译：结肠链霉菌 sp.。 nov。，从马来西亚红树林土壤中分离出来的新型放线菌，具有抗人结肠癌细胞系的抗氧化活性和细胞毒性潜力

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摘要

Streptomyces colonosanans MUSC 93J~(T), a novel strain isolated from mangrove forest soil located at Sarawak, Malaysia. The bacterium was noted to be Gram-positive and to form light yellow aerial and vivid yellow substrate mycelium on ISP 2 agar. The polyphasic approach was used to determine the taxonomy of strain MUSC 93J~(T)and the strain showed a range of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces . Phylogenetic and 16S rRNA gene sequence analysis indicated that closely related strains include Streptomyces malachitofuscus NBRC 13059~(T)(99.2% sequence similarity), Streptomyces misionensis NBRC 13063~(T)(99.1%), and Streptomyces phaeoluteichromatogenes NRRL 5799~(T)(99.1%). The DNA–DNA relatedness values between MUSC 93J~(T)and closely related type strains ranged from 14.4 ± 0.1 to 46.2 ± 0.4%. The comparison of BOX-PCR fingerprints indicated MUSC 93J~(T)exhibits a unique DNA profile. The genome of MUSC 93J~(T)consists of 7,015,076 bp. The DNA G + C content was determined to be 69.90 mol%. The extract of strain MUSC 93J~(T)was demonstrated to exhibit potent antioxidant activity via ABTS, metal chelating, and SOD assays. This extract also exhibited anticancer activity against human colon cancer cell lines without significant cytotoxic effect against human normal colon cells. Furthermore, the chemical analysis of the extract further emphasizes the strain is producing chemo-preventive related metabolites. Based on this polyphasic study of MUSC 93J~(T), it is concluded that this strain represents a novel species, for which the name Streptomyces colonosanans sp. nov. is proposed. The type strain is MUSC 93J~(T)(= DSM 102042~(T)= MCCC 1K02298~(T)).
机译：结肠链霉菌MUSC 93J〜（T），一种新菌株，是从位于马来西亚砂拉越的红树林森林土壤中分离出来的。注意到该细菌为革兰氏阳性，并在ISP 2琼脂上形成了淡黄色的气生菌和鲜黄色的底物菌丝体。多相法用于确定菌株MUSC 93J〜（T）的分类，该菌株显示出与链霉菌属成员一致的一系列系统发育和化学分类特性。系统发育和16S rRNA基因序列分析表明，密切相关的菌株包括疟原虫链霉菌NBRC 13059〜（T）（99.2％序列相似性），miseptensis米非链霉菌NBRC 13063〜（T）（99.1％）和嗜盐链霉菌NRRL 5799〜（T）。 （99.1％）。 MUSC 93J〜（T）与密切相关的菌株之间的DNA-DNA相关性值介于14.4±0.1至46.2±0.4％之间。 BOX-PCR指纹图谱的比较表明MUSC 93J〜（T）表现出独特的DNA谱。 MUSC 93J〜（T）的基因组由7,015,076 bp组成。测定的DNA G + C含量为69.90mol％。通过ABTS，金属螯合和SOD分析，证明MUSC 93J〜（T）菌株的提取物显示出有效的抗氧化活性。该提取物还显示出对人结肠癌细胞系的抗癌活性，而对人正常结肠细胞没有明显的细胞毒性作用。此外，提取物的化学分析进一步强调了该菌株正在产生化学预防相关的代谢产物。基于对MUSC 93J〜（T）的多相研究，可以得出结论，该菌株代表了一种新物种，其名称为Streptomyces colonosanans sp。十一月被提议。类型菌株为MUSC 93J〜（T）（= DSM 102042〜（T）= MCCC 1K02298〜（T））。

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