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首页> 外文期刊>Frontiers in Neuroanatomy >Spatial and Temporal Distribution of Dopaminergic Neurons during Development in Zebrafish
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Spatial and Temporal Distribution of Dopaminergic Neurons during Development in Zebrafish

机译：斑马鱼发育过程中多巴胺能神经元的时空分布

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As one of the model organisms of Parkinson’s disease (PD) research, the zebrafish has its advantages, such as the 87% homology with human genome and transparent embryos which make it possible to observe the development of dopaminergic neurons in real time. However, there is no midbrain dopaminergic system in zebrafish when compared with mammals, and the location and projection of the dopaminergic neurons are seldom reported. In this study, Vmat2:GFP transgenic zebrafish was used to observe the development and distribution of dopaminergic neurons in real time. We found that diencephalons (DC) 2 and DC4 neuronal populations were detected at 24 h post fertilization (hpf). All DC neuronal populations as well as those in locus coeruleus (LC), raphe nuclei (Ra) and telencephalon were detected at 48 hpf. Axons were detected at 72 hpf. At 96 hpf, all the neuronal populations were detected. For the first time we reported axons from the posterior tubercle (PT) of ventral DC projected to subpallium in vivo . However, when compared with results from whole mount tyrosine hydroxylase (TH) immunofluorescence staining in wild type (WT) zebrafish, we found that DC2 and DC4 neuronal populations were mainly dopaminergic, while DC1, DC3, DC5 and DC6 might not. Neurons in pretectum (Pr) and telencephalon were mainly dopaminergic, while neurons in LC and Ra might be noradrenergic. Our study makes some corrections and modifications on the development, localization and distribution of zebrafish dopaminergic neurons, and provides some experimental evidences for the construction of the zebrafish PD model.
机译：作为帕金森氏病（PD）研究的典范生物之一，斑马鱼具有其优势，例如与人类基因组的87％同源性和透明的胚胎，使得可以实时观察多巴胺能神经元的发育。但是，与哺乳动物相比，斑马鱼中没有中脑多巴胺能系统，很少报道多巴胺能神经元的位置和投射。在这项研究中，使用Vmat2：GFP转基因斑马鱼实时观察多巴胺能神经元的发育和分布。我们发现在受精（hpf）后24小时检测到了间脑（DC）2和DC4神经元种群。在48 hpf时检测到所有DC神经元种群，以及蓝斑脑（LC），缝核（Ra）和端脑的神经元。在72 hpf处检测到轴突。在96 hpf时，检测到所有神经元种群。我们首次报道了体内DC的后结节（PT）的轴突投射到大脑皮层下。但是，与野生型（WT）斑马鱼的全装载酪氨酸羟化酶（TH）免疫荧光染色的结果相比，我们发现DC2和DC4神经元群体主要是多巴胺能，而DC1，DC3，DC5和DC6可能不是。前保护区（Pr）和端脑中的神经元主要是多巴胺能，而LC和Ra中的神经元可能是去甲肾上腺素能。我们的研究对斑马鱼多巴胺能神经元的发育，定位和分布进行了一些修正和修改，为斑马鱼PD模型的建立提供了实验依据。

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