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首页> 外文期刊>Frontiers in Plant Science >Molecular Cytogenetic Characterization of New Wheat— Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat
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Molecular Cytogenetic Characterization of New Wheat— Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat

机译：改良小麦籽粒品质的新小麦-<斜纹>达斯麦基因渗入系的分子细胞遗传学表征

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As an important relative of wheat ( Triticum aestivum L), Dasypyrum breviaristatum contains novel high molecular weight glutenin subunits (HMW-GSs) encoded by Glu-1Vb genes. We identified new wheat— D. breviaristatum chromosome introgression lines including chromosomes 1V~(b)and 1V~(b)L.5V~(b)L by fluorescence in situ hybridization (FISH) combined with molecular markers. We found that chromosome changes occurred in the wheat— D. breviaristatum introgression lines and particularly induced the deletion of 5BS terminal repeats and formation of a new type of 5B-7B reciprocal translocation. The results imply that the D. breviaristatum chromosome 1V~(b)may contain genes which induce chromosomal recombination in wheat background. Ten putative high molecular weight glutenin subunit (HMW-GS) genes from D. breviaristatum and wheat— D. breviaristatum introgression lines were isolated. The lengths of the HMW-GS genes in Dasypyrum were significantly shorter than typical HMW-GS of common wheat. A new y-type HMW-GS gene, named Glu-Vb1y , was characterized in wheat— D. breviaristatum 1V~(b)introgression lines. The new wheat— D. breviaristatum germplasm displayed reduced plant height, increased tillers and superior grain protein and gluten contents, improved gluten performance index. The results showed considerable potential for utilization of D. breviaristatum chromosome 1V~(b)segments in future wheat improvement.
机译：作为小麦的重要亲缘种，短叶稻草（Dasypyrum breviaristatum）包含由Glu-1Vb基因编码的新型高分子量谷蛋白亚基（HMW-GSs）。通过荧光原位杂交（FISH）结合分子标记，我们鉴定出了新的小麦—D。breviaristatum染色体基因渗入系，包括1V〜（b）和1V〜（b）L.5V〜（b）L染色体。我们发现，染色体变化发生在小麦—短鞭毛虫（D.breviaristatum）基因渗入系中，并特别诱导了5BS末端重复序列的缺失和新型5B-7B相互易位的形成。结果表明，D.breviaristatum染色体1V〜（b）可能包含在小麦背景中诱导染色体重组的基因。分离了来自短叶杜鹃和小麦-短叶杜鹃渗入系的十个推定的高分子量谷蛋白亚基（HMW-GS）基因。稻瘟病菌中HMW-GS基因的长度明显短于普通小麦的典型HMW-GS。在小麦D.breviaristatum 1V〜（b）基因渗入系中鉴定了一个新的y型HMW-GS基因，称为Glu-Vb1y。新的小麦D. breviaristatum种质显示出降低的株高，增加的分till和优质的谷蛋白和面筋含量，改善了面筋性能指数。结果表明，D.breviaristatum 1V〜（b）染色体片段在小麦改良中具有很大的潜力。

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