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首页> 外文期刊>Frontiers in Plant Science >Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers
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Drought Tolerance in Pinus halepensis Seed Sources As Identified by Distinctive Physiological and Molecular Markers

机译：独特生理和分子标记鉴定的 Palus halepensis 种子来源的耐旱性

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Drought is one of the main constraints determining forest species growth, survival and productivity, and therefore one of the main limitations for reforestation or afforestation. The aim of this study is to characterize the drought response at the physiological and molecular level of different Pinus halepensis (common name Aleppo pine) seed sources, previously characterized in field trials as drought-sensitive or drought-tolerant. This approach aims to identify different traits capable of predicting the ability of formerly uncharacterized seedlings to cope with drought stress. Gas-exchange, water potential, photosynthetic pigments, soluble sugars, free amino acids, glutathione and proteomic analyses were carried out on control and drought-stressed seedlings in greenhouse conditions. Gas-exchange determinations were also assessed in field-planted seedlings in order to validate the greenhouse experimental conditions. Drought-tolerant seed sources presented higher values of photosynthetic rates, water use efficiency, photosynthetic pigments and soluble carbohydrates concentrations. We observed the same pattern of variation of photosynthesis rate and maximal efficiency of PSII in field. Interestingly drought-tolerant seed sources exhibited increased levels of glutathione, methionine and cysteine. The proteomic profile of drought tolerant seedlings identified two heat shock proteins and an enzyme related to methionine biosynthesis that were not present in drought sensitive seedlings, pointing to the synthesis of sulfur amino acids as a limiting factor for drought tolerance in Pinus halepensis . Our results established physiological and molecular traits useful as distinctive markers to predict drought tolerance in Pinus halepensis provenances that could be reliably used in reforestation programs in drought prone areas.
机译：干旱是决定森林物种生长，存活和生产力的主要限制因素之一，因此也是造林或造林的主要限制之一。这项研究的目的是在生理和分子水平上表征不同类型的halusensis（俗称阿勒颇松）种子源的干旱反应，该种子源以前在田间试验中被描述为对干旱敏感或耐旱的种子。该方法旨在确定能够预测以前未鉴定的幼苗应对干旱胁迫能力的不同性状。在温室条件下，对对照和干旱胁迫幼苗进行了气体交换，水势，光合色素，可溶性糖，游离氨基酸，谷胱甘肽和蛋白质组学分析。还对田间种植的幼苗中的气体交换测定进行了评估，以验证温室实验条件。耐旱种子源具有更高的光合速率，水分利用效率，光合色素和可溶性碳水化合物的含量。我们在田间观察到相同的光合作用速率变化和PSII最大效率的变化模式。有趣的是，耐旱种子源的谷胱甘肽，蛋氨酸和半胱氨酸水平升高。耐旱幼苗的蛋白质组学特征鉴定出了两种热休克蛋白和一种与蛋氨酸生物合成有关的酶，这些蛋白在干旱敏感幼苗中不存在，这表明合成硫氨基酸是限制樟子松耐旱性的限制因素。我们的研究结果建立了生理和分子特性，可作为预测樟子松种源耐旱性的独特标志，可在干旱多发地区的造林计划中可靠使用。

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