...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Plant Science >Identification and Comparative Analysis of Differential Gene Expression in Soybean Leaf Tissue under Drought and Flooding Stress Revealed by RNA-Seq
【24h】

Identification and Comparative Analysis of Differential Gene Expression in Soybean Leaf Tissue under Drought and Flooding Stress Revealed by RNA-Seq

机译：RNA-Seq揭示的干旱和淹水胁迫下大豆叶片组织中差异基因表达的鉴定与比较分析

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Drought and flooding are two major causes of severe yield loss in soybean worldwide. A lack of knowledge of the molecular mechanisms involved in drought and flood stress has been a limiting factor for the effective management of soybeans; therefore, it is imperative to assess the expression of genes involved in response to flood and drought stress. In this study, differentially expressed genes (DEGs) under drought and flooding conditions were investigated using Illumina RNA-Seq transcriptome profiling. A total of 2724 and 3498 DEGs were identified under drought and flooding treatments, respectively. These genes comprise 289 Transcription Factors (TFs) representing Basic Helix-loop Helix (bHLH), Ethylene Response Factors (ERFs), myeloblastosis (MYB), No apical meristem (NAC), and WRKY amino acid motif (WRKY) type major families known to be involved in the mechanism of stress tolerance. The expression of photosynthesis and chlorophyll synthesis related genes were significantly reduced under both types of stresses, which limit the metabolic processes and thus help prolong survival under extreme conditions. However, cell wall synthesis related genes were up-regulated under drought stress and down-regulated under flooding stress. Transcript profiles involved in the starch and sugar metabolism pathways were also affected under both stress conditions. The changes in expression of genes involved in regulating the flux of cell wall precursors and starch/sugar content can serve as an adaptive mechanism for soybean survival under stress conditions. This study has revealed the involvement of TFs, transporters, and photosynthetic genes, and has also given a glimpse of hormonal cross talk under the extreme water regimes, which will aid as an important resource for soybean crop improvement.
机译：干旱和洪水是造成世界范围内大豆严重减产的两个主要原因。对干旱和洪水胁迫涉及的分子机制的了解不足已成为有效管理大豆的限制因素。因此，必须评估与洪水和干旱胁迫有关的基因的表达。在这项研究中，使用Illumina RNA-Seq转录组分析对干旱和洪水条件下的差异表达基因（DEG）进行了研究。在干旱和洪水处理下，总共鉴定出2724和3498 DEG。这些基因包含289个转录因子（TF），分别代表基本螺旋环螺旋（bHLH），乙烯反应因子（ERF），成纤维细胞形成（MYB），无顶端分生组织（NAC）和WRKY氨基酸基序（WRKY）类型的主要家族参与压力耐受机制。在两种胁迫下，光合作用和叶绿素合成相关基因的表达均显着降低，这限制了代谢过程，从而有助于延长极端条件下的存活。然而，细胞壁合成相关基因在干旱胁迫下被上调而在洪水胁迫下被下调。在两种胁迫条件下，参与淀粉和糖代谢途径的转录物谱也受到影响。参与调节细胞壁前体通量和淀粉/糖含量的基因表达变化可以作为大豆在胁迫条件下存活的适应机制。这项研究揭示了TF，转运蛋白和光合作用基因的参与，并且还瞥见了极端水分条件下的荷尔蒙串扰，这将成为大豆改良的重要资源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号