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首页> 外文期刊>Frontiers in Public Health >Limpet (Scutellastra cochlear) Recovered From Some Estuaries in the Eastern Cape Province, South Africa Act as Reservoirs of Pathogenic Vibrio Species
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Limpet (Scutellastra cochlear) Recovered From Some Estuaries in the Eastern Cape Province, South Africa Act as Reservoirs of Pathogenic Vibrio Species

机译：从南非东开普省的一些河口中发现的（Scutellastra耳蜗）充当病原性弧菌物种的贮藏库

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Limpet (Scutellastra cochlear) serves as seafood recipe and an important member of the aquatic food chain. It is an abundant mollusc in some aquatic environment in South Africa. In this study, we investigated the potential of the molluscs harvested from the Buffalo, Swartkops, and Kowie estuaries in the Eastern Cape Province, South Africa to serve as transient or maintenance reservoir of Vibrio species. The mollusc and source water samples were collected monthly from the rivers between December 2016 and November 2017. The reservoir category of the limpet samples recovered was determined by employing the combination of MPN-PCR method and statistical analysis (comparison of mean and proportion tests). The densities of Vibrio spp. in limpet and their source water samples were determined using MPN-PCR methods. Presumptive isolates were recovered by processing the samples with thiosulfate-citrate-bile salts-sucrose agar and where necessary, samples were enriched with alkaline peptone water. The presumptive isolates were identified using PCR methods with emphasis on six Vibrio species of public health importance. Vibrio spp. were detected in all the limpet samples but not in all the water samples. The densities of Vibrio spp. in the limpet samples were more than the densities of Vibrio species in their source water and these were significant at P < 0.05. In like manner, five out of the six key Vibrio pathogens targeted in this study were more prevalent in limpet samples than in source water samples. Based on our findings, we concluded that our method though could be improved on, is efficient for the determination of reservoir types of bacterial-carrying organisms. We also concluded that the limpet found in the estuaries are not just a transient but a maintenance reservoir of Vibrio spp. which could cause vibrio-related infections.
机译：m（Scutellastra耳蜗）是海鲜食谱，也是水生食物链的重要成员。在南非的某些水生环境中，它是一种丰富的软体动物。在这项研究中，我们调查了从南非东开普省的布法罗，Swartkops和Kowie河口收获的软体动物作为弧菌物种的暂存库或维持库的潜力。在2016年12月至2017年11月期间，每月从河流中采集软体动物和水源样品。回收的帽贝样品的水库类别通过MPN-PCR方法与统计分析（均值和比例测试的比较）相结合来确定。弧菌的密度。使用MPN-PCR方法测定了衣壳中的及其来源水样品。通过用硫代硫酸盐-柠檬酸盐-胆汁盐-蔗糖琼脂处理样品来回收推测的分离物，并在必要时用碱性蛋白ept水富集样品。使用PCR方法鉴定了推定的分离株，重点是对六个具有公共卫生重要性的弧菌。弧菌在所有帽贝样品中都检测到了，但是在所有水样中都没有检测到。弧菌的密度。帽贝样品中的弧菌比其源水中的弧菌物种密度高，且在P <0.05时显着。同样，在本研究中靶向的六个关键弧菌病原体中有五个在帽贝样品中比在源水样品中更普遍。根据我们的发现，我们得出结论，尽管我们的方法可以改进，但对于确定细菌携带生物的储库类型是有效的。我们还得出结论，在河口发现的帽贝不仅是暂时的，而且是弧菌的维持库。可能导致弧菌相关感染。

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