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首页> 外文期刊>Functional Foods In Health And Disease >Redox-dependent and independent mechanisms of selective pro- and anti-apoptotic activity of Georgian legumes crops extracts on Jurkat and MDCK cells
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Redox-dependent and independent mechanisms of selective pro- and anti-apoptotic activity of Georgian legumes crops extracts on Jurkat and MDCK cells

机译：佐治亚豆类作物提取物对Jurkat和MDCK细胞的选择性促凋亡和抗凋亡活性的氧化还原依赖性和独立机制

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Background: Possibilities of pharmacological regulation of apoptosis play an important role in the treatment of different diseases. Polyphenol-rich plant extracts as well as isolated polyphenols can regulate cell apoptosis primarily through intrinsic and extrinsic mechanisms of action and are the most intriguing and studied class of compounds that can be therapeutics for a wide range of common diseases, including cancer. Polyphenols are well known as powerful antioxidants, and their action is also associated with pro-apoptotic function in various types of tumor cells.The purpose of this study was to establish the anti-and pro-apoptotic activity of Georgian legume crops in model cellular systems.Methods: Legume crop extracts (LCEs) were prepared in water-alcohol solute. Polyphenol content in the extracts was determined using the Folin–Ciocalteu method; antiradical activity (AA) - according the comparative time of the 50% neutralization of 2,2-diphenyl-1-picrylhydrazyl (DPPH) cleavage.Studies were carried out on human leukemic mature T cells (Jurkat) and normal epithelial MDCK cell lines. For modelling of oxidative stress, 30% hydrogen peroxide was used. LCEs were added to intact or incubated Jurkat and MDCK cells under oxidative stress conditions. Cell viability was determined by 3-(4,5-dimethyltiazol-2)-2,5-diphenyl-tetrazolium-bromide (MTT) test. Catalase and Superoxidedismutase (SOD) activity in cellular supernatant was measured byspectrophotometry.Results: LCEs revealed selective pro- and antiapoptotic activity on the intact and incubated cells under oxidative stress conditions Jurkat and MDCK cells. The cytotoxic effect of LCEs on intact Jurkat and MDCK cells was independent of their AA and activity of enzymatic cellular antioxidant defense system.The cytoprotective effect of LCEs on MDCK cells was realized through redox-dependent mechanisms and is associated both with the own AA of the extracts and with the stimulating effect of the extracts on enzymatic cellular antioxidant defense system activity, where catalase played a leading role. LCEs didn’t protect Jurkat cells from oxidative stress-induced apoptosis.Conclusion: The obtained results allow us to conclude that in cancer chemotherapy, the legume extracts might be combined with prooxidant drugs in order to protect normal cells, but not malignant ones, from their apoptosis-inducing effect. On the other hand, these extracts may protect non- malignant tissues/organs from various apoptosis-related disorders.
机译：背景：凋亡的药理学调节可能在不同疾病的治疗中起着重要作用。富含多酚的植物提取物以及分离出的多酚可以主要通过内在和外在的作用机制来调节细胞凋亡，并且是最吸引人和研究最多的一类化合物，可以用于治疗多种常见疾病，包括癌症。多酚是众所周知的强大抗氧化剂，其作用还与各种类型肿瘤细胞中的促凋亡功能有关。本研究的目的是建立模型细胞系统中格鲁吉亚豆类作物的抗凋亡和促凋亡活性。方法：在水-醇溶质中制备豆类作物提取物（LCEs）。提取物中的多酚含量采用Folin–Ciocalteu方法测定;抗自由基活性（AA）-根据50％中和2,2-二苯基-1-吡啶并肼基（DPPH）裂解的比较时间进行研究。研究涉及人类白血病成熟T细胞（Jurkat）和正常上皮MDCK细胞系。为了模拟氧化应激，使用了30％的过氧化氢。在氧化应激条件下，将LCE加入完整或孵育的Jurkat和MDCK细胞中。细胞存活力通过3-（4,5-二甲基噻唑-2）-2,5-二苯基-溴化四唑（MTT）测试确定。分光光度法测定细胞上清液中的过氧化氢酶和超氧化物歧化酶（SOD）活性。结果：LCEs在氧化应激条件下，Jurkat和MDCK细胞对完整和孵育的细胞具有选择性的促凋亡和抗凋亡活性。 LCEs对完整Jurkat和MDCK细胞的细胞毒作用与它们的AA和酶促细胞抗氧化防御系统的活性无关。提取物并具有提取物对酶促细胞抗氧化剂防御系统活性的刺激作用，其中过氧化氢酶起主导作用。 LCEs不能保护Jurkat细胞免受氧化应激诱导的细胞凋亡。结论：所得结果可以使我们得出结论，在癌症化学疗法中，豆类提取物可能与促氧化剂结合使用，以保护正常细胞免受恶性肿瘤侵袭。它们的凋亡诱导作用。另一方面，这些提取物可以保护非恶性组织/器官免于各种细胞凋亡相关的疾病。

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