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首页> 外文期刊>Functional Foods In Health And Disease >Anti-inflammatory and anti-allergic activities of Skipjack tuna (Katsuwonus pelamis) dark muscle hydrolysates evaluated in cell culture model
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Anti-inflammatory and anti-allergic activities of Skipjack tuna (Katsuwonus pelamis) dark muscle hydrolysates evaluated in cell culture model

机译：在细胞培养模型中评估Skipjack金枪鱼（Katsuwonus pelamis）黑肌肉水解产物的抗炎和抗过敏活性

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Background: Oxidative stress and inflammation are inextricably linked and play major roles in the onset and development of Non-communicable diseases (NCD) which are the most common cause of death and disability in modern world. Hydrolyzed proteins have also been suggested to be used to manage adverse food allergic reaction. Therefore, this study aimed to investigate anti-inflammatory and anti-allergy activities of dark muscle tuna hydrolysates using biological cell line systems as a function of enzyme, the extent of hydrolysis and molecular weight range.Methods: Dark muscle tuna hydrolysates were prepared with two different enzyme types; Alcalase and Flavourzyme. Anti-inflammation activity was measured by inhibitory effect of nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Anti-allergy was determined from ability of hydrolysates to inhibit ? -hexsosaminidase (-HEX) release from RBL-2H3 mast cells. Cytotoxicity was also investigated in both RAW 264.7 macrophage cells and RBL-2H3 mast cells.Results: No cytotoxic effect on RAW 264.7 macrophage cells and RBL-2H3 mast cells was observed. The NO inhibition and ? -HEX release were found significant in dose dependent manner (p<0.05). Alcalase hydrolysates demonstrated greater anti-inflammatory and anti-allergic activities than Flavourzyme hydrolysates (p 45.44 ?g/ml for NO inhibition and > 65.23 ?g/ml for ? -HEX release inhibition. These effects increased with the extent of hydrolysis and enzyme concentration. The peptide of lowest molecular weight range (< 3 KDa) was highest in anti-inflammatory and anti-allergic actions. Reducing secretion of TNF-?, IL-6 and IL-1? was found greater in Alcalase hydrolysate than Flavourzyme one.Conclusions: Skipjack tuna dark muscle hydrolysates from Alcalase resulted in peptides with anti-inflammation activity, as determined by NO production in LPS-stimulated RAW 264.7 macrophage cells and anti-allergic properties as measured by a suppression of degranulation of sensitized RBL-2H3 cells. Anti-inflammatory effect may be due to their anti-oxidative capacity and relevant inflammatory factors attenuated with hydrolysate by reducing secretion of pro-inflammatory cytokine (TNF-?, IL-6 and IL-1?). Inhibition of ? -HEX release by peptides may be due to membrane-stabilizing action or/and blockade of IgE antibody at fragment region.
机译：背景：氧化应激和炎症之间有着千丝万缕的联系，并在非传染性疾病（NCD）的发生和发展中起着重要作用，这些疾病是现代世界最常见的死亡和残疾原因。还已经建议将水解蛋白用于处理不利的食物过敏反应。因此，本研究旨在利用生物细胞系对酶，水解程度和分子量范围的影响，研究黑肌金枪鱼水解产物的抗炎和抗过敏活性。方法：用两种方法制备黑肌金枪鱼水解产物。不同的酶类型; Alcalase和Flavourzyme。通过一氧化氮（NO）产生对脂多糖（LPS）刺激的RAW 264.7巨噬细胞的抑制作用来测量抗炎活性。从水解产物抑制β-淀粉样蛋白的能力确定抗过敏性。 -己糖胺酶（-HEX）从RBL-2H3肥大细胞中释放。还对RAW 264.7巨噬细胞和RBL-2H3肥大细胞均进行了细胞毒性研究。结果：未观察到对RAW 264.7巨噬细胞和RBL-2H3肥大细胞有细胞毒性作用。 NO抑制和？发现-HEX释放具有剂量依赖性（p <0.05）。 Alcalase水解产物显示出比Flavourzyme水解产物更高的抗炎和抗过敏活性（抑制NO的p为45.44μg/ ml，抑制β-HEX释放的> 65.23μg/ ml。这些作用随水解程度和酶浓度的增加而增加分子量范围最低（<3 KDa）的肽具有最高的抗炎和抗过敏作用，在Alcalase水解产物中发现的TNF-α，IL-6和IL-1β的减少分泌要比Flavourzyme 1更大。结论：Alcalase的Ski鱼金枪鱼深色肌肉水解产物可产生具有抗炎活性的肽，如通过LPS刺激的RAW 264.7巨噬细胞中NO的产生所确定的，以及通过抑制致敏的RBL-2H3细胞脱粒所测量的抗过敏特性。抗炎作用可能是由于它们的抗氧化能力和相关的炎性因子被水解产物通过减少促炎性细胞因子（TNF-α，IL-6 a nd IL-1？）。抑制？肽释放的-HEX可能是由于膜稳定作用或/和IgE抗体在片段区域的阻滞所致。

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