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首页> 外文期刊>Marine ecology progress series >Widespread detection of circular replication initiator protein (rep)-encoding ssDNA viral genomes in estuarine, coastal and open ocean net plankton
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Widespread detection of circular replication initiator protein (rep)-encoding ssDNA viral genomes in estuarine, coastal and open ocean net plankton

机译：在河口，沿海和开放海洋网浮游生物中广泛检测编码环状复制起始蛋白（rep）的ssDNA病毒基因组

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ABSTRACT: Circular replication initiator protein (rep)-encoding ssDNA (CRESS-DNA) viruses have been widely reported in viral metagenomic surveys and in association with invertebrate zooplankton in freshwater and marine habitats. However, there have been no systematic or quantitative studies of their distribution in marine waters. We investigated the distribution of CRESS-DNA viruses in net plankton (64 μm) collected from geographically widespread locations, using quantitative PCR that targets viral genotypes previously recovered from soil, freshwater and estuarine free-living viruses, and viruses associated with arthropod tissues. We detected CRESS-DNA viruses in most net plankton samples except for a sample containing only the cyanobacterium Trichodesmium. Soil and freshwater plankton CRESS-DNA viruses were detected only at sites with substantial freshwater and runoff effects, while 2 CRESS-DNA viruses recovered from plankton of the Chesapeake Bay were detected in most net plankton tested. CRESS-DNA viruses recovered from marine copepods, the freshwater cladoceran Daphnia and the freshwater amphipod Diporeia were primarily detected in habitats where similar hosts were observed in zooplankton counts. Our data suggest that CRESS-DNA viruses previously recovered from invertebrate tissues and from virioplankton may be widely distributed in plankton 64 μm, providing evidence for a zooplankton origin of this viral group.
机译：摘要：在病毒宏基因组学研究中以及与淡水和海洋生境中的无脊椎动物浮游生物相关的报道中，广泛报道了编码环状复制起始蛋白（ rep ）的ssDNA（CRESS-DNA）病毒。但是，还没有系统或定量研究其在海水中的分布。我们使用定量PCR技术研究了从地理分布广泛的地区收集的净浮游生物（> 64μm）中CRESS-DNA病毒的分布，该PCR的目标是先前从土壤，淡水和河口自由生活病毒以及与节肢动物组织相关的病毒中回收的病毒基因型。除了仅包含蓝细菌 Trichodesmium 的样本外，我们在大多数净浮游生物样本中检测到CRESS-DNA病毒。仅在具有大量淡水和径流影响的地点检测到土壤和淡水浮游生物CRESS-DNA病毒，而在大多数测试的净浮游生物中检测到2种从切萨皮克湾浮游生物回收的CRESS-DNA病毒。从海洋pe足类动物，淡水枝角类鱼类“水蚤”和淡水两栖纲动物“双孢子虫”中回收的CRESS-DNA病毒主要在浮游动物计数中观察到类似寄主的生境中检测到。我们的数据表明，先前从无脊椎动物组织和浮游动物中回收的CRESS-DNA病毒可能广泛分布在> 64μm的浮游生物中，为该病毒群的浮游动物起源提供了证据。

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