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首页> 外文期刊>Mass Spectrometry Letters >A Sensitive and Rapid LC-MS/MS Method for Determination of Berberine in Human Plasma
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A Sensitive and Rapid LC-MS/MS Method for Determination of Berberine in Human Plasma

机译：快速灵敏的LC-MS / MS法测定人血浆中的小Ber碱

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摘要

Coptidis Rhizoma (CR) has been used widely in traditional medicine to treat common diseases. This study aimed to develop a high-sensitivity liquid chromatography-tandem mass (LC-MS) spectrometry method for the evaluation of the pharmacokinetics of a new natural product that contain CR extract with the main bioactive compound, berberine, at trace concentrations. Human plasma samples were pretreated with methanol by a protein precipitation method. Berberine was analyzed on a Kinetex C18 column ( $2.1mm{imes}50mm$ , $100{AA}$ , $1.7{mu}m$ ) using a mobile phase of 10 mM ammonium formate/0.1% formic acid in water (A) and acetonitrile (B) (50:50, v/v) with a flow rate of 0.25 mL/min. The analyte was detected by using electrospray ionization in positive mode with multiple reaction monitoring (MRM). The method was sensitive, with a lower limit of quantification of 1 pg/mL, which has not been previously obtained. The method was validated (over the range of 1-50 pg/mL) and applied successfully for the pharmacokinetic study of human plasma samples.
机译：黄连（CR）已在传统医学中广泛用于治疗常见疾病。这项研究的目的是开发一种高灵敏度液相色谱-串联质谱（LC-MS）光谱法，用于评估一种新的天然产物的药代动力学，该天然产物中含有CR提取物和主要的生物活性化合物小trace碱，且含量低。通过蛋白质沉淀法用甲醇预处理人血浆样品。在Kinetex C18柱上分析小碱（2.1mm { times} 50mm $，$ 100 { AA} $，$ 1.7 { mu} m $），使用10 mM甲酸铵/0.1%甲酸水溶液的流动相（ A）和乙腈（B）（50:50，v / v），流速为0.25 mL / min。通过使用正离子模式下的电喷雾电离和多反应监测（MRM）来检测分析物。该方法灵敏，其定量下限为1 pg / mL，这是以前无法获得的。该方法经过验证（在1-50 pg / mL的范围内），并成功地用于人体血浆样品的药代动力学研究。

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