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首页> 外文期刊>Mass Spectrometry Letters >Simultaneous Quantitative Determination of Nine Hallucinogenic NBOMe Derivatives in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry
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Simultaneous Quantitative Determination of Nine Hallucinogenic NBOMe Derivatives in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry

机译：液相色谱-串联质谱法同时定量测定人血浆中的九种致幻性NBOMe衍生物

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摘要

We developed a bioanalytical method for simultaneous determination of nine NBOMe derivatives (25H-NBOMe, 25B-NBOMe, 25E-NBOMe, 25N-NBOMe, 25C-NBOH, 25I-NBOH, 25B-NBF, 25C-NBF, and 25I-NBF) in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Human plasma samples were pre-treated using solid-phase extraction. Separation was achieved on a C18 column under gradient elution using a mobile phase containing 0.1% formic acid in acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Mass detection was performed in the positive ion mode using multiple reaction monitoring. The calibration range was 1-100 ng/mL for all quantitative analytes, with a correlation coefficient greater than 0.99. The intra- and inter-day precision and accuracy varied from 0.85 to 6.92% and from 90.19 to 108.69%, respectively. The recovery ranged from 86.36 to 118.52%, and the matrix effects ranged from 27.09 to 99.72%. The stability was acceptable in various conditions. The LC-MS/MS method was validated for linearity, accuracy, precision, matrix effects, recovery and stability in accordance with the FDA guidance. The proposed method is suitable for reliable and robust routine screening and analysis of nine NBOMe derivatives in forensic field.
机译：我们开发了一种生物分析方法，可同时测定9种NBOMe衍生物（25H-NBOMe，25B-NBOMe，25E-NBOMe，25N-NBOMe，25C-NBOH，25I-NBOH，25B-NBF，25C-NBF和25I-NBF）液相色谱串联质谱法（LC-MS / MS）测定人血浆中的维生素E。使用固相萃取对人血浆样品进行预处理。在C18色谱柱上以0.3 mL / min的流速，使用含有0.1％甲酸的乙腈溶液和0.1％甲酸的水溶液的流动相，在梯度洗脱下实现分离。使用多反应监测以阳离子模式进行质量检测。所有定量分析物的校准范围为1-100 ng / mL，相关系数大于0.99。日内和日间精度和准确性分别从0.85％到6.92％和90.19％到108.69％不等。回收率从86.36％到118.52％，基质效应从27.09％到99.72％。在各种条件下稳定性是可接受的。 LC-MS / MS方法已根据FDA指南验证了线性，准确性，精密度，基质效应，回收率和稳定性。该方法适用于法医领域中九种NBOMe衍生物的可靠，稳定的常规筛选和分析。

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