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首页> 外文期刊>Materials >Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser
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Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser

机译：使用940 nm二极管激光器对培养的人成纤维细胞进行生物刺激

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Background: Fibroblasts are the main cells involved in regeneration during wound healing. The objective was to determine the effect of 940 nm diode laser on cultured human fibroblasts using different irradiation regimens. Methods: The CCD-1064Sk human epithelial fibroblast cell line was treated with a 940 nm diode laser at different energy doses (power: 0.2–1 W and energy density: 1–7 J/cm 2 ) using different transmission modes (continuous or pulsed). The effect on cell growth at 24 and 72 h post-treatment was examined by measuring the proliferative capacity, the impact on the cell cycle, and the effect on cell differentiation. Results: fibroblast proliferative capacity was increased at 24 and 72 h post-treatment as a function of the energy dose. The greatest increase was observed with a power of 0.2 or 0.5 W and energy density between 1 and 4 J/cm 2 ; no difference was observed between continuous and pulsed modes. There were no significant differences in cell cycle between treated groups and controls. α-actin expression was increased by treatment, indicating enhanced cell differentiation. Conclusion: The 940 nm diode laser has biostimulating effects on fibroblasts, stimulating proliferative capacity and cell differentiation without altering the cell cycle. Further researches are necessary to explore its potential clinical usefulness in wound healing.
机译：背景：成纤维细胞是伤口愈合过程中参与再生的主要细胞。目的是确定使用不同的照射方案，940 nm二极管激光对培养的人成纤维细胞的影响。方法：使用不同的传输模式（连续或脉冲），用940 nm二极管激光器以不同的能量剂量（功率：0.2–1 W和能量密度：1–7 J / cm 2）处理CCD-1064Sk人上皮成纤维细胞系）。通过测量增殖能力，对细胞周期的影响以及对细胞分化的影响，检查了在处理后24和72 h对细胞生长的影响。结果：成纤维细胞的增殖能力在治疗后24和72 h随能量剂量的增加而增加。当功率为0.2或0.5 W且能量密度为1-4 J / cm 2时，观察到最大的增加;在连续模式和脉冲模式之间未观察到差异。治疗组和对照组之间的细胞周期没有显着差异。通过治疗，α-肌动蛋白表达增加，表明细胞分化增强。结论：940 nm二极管激光器对成纤维细胞具有生物刺激作用，在不改变细胞周期的情况下刺激其增殖能力和细胞分化。有必要进行进一步的研究以探索其在伤口愈合中的潜在临床实用性。

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