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首页> 外文期刊>Materials >Optimal Structure of a Plasmonic Chip for Sensitive Bio-Detection with the Grating-Coupled Surface Plasmon-Field Enhanced Fluorescence (GC-SPF)
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Optimal Structure of a Plasmonic Chip for Sensitive Bio-Detection with the Grating-Coupled Surface Plasmon-Field Enhanced Fluorescence (GC-SPF)

机译：光栅耦合的表面等离激元场增强荧光（GC-SPF）用于敏感生物检测的等离激元芯片的最佳结构。

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摘要

Surface plasmon field-enhanced fluorescence (SPF) has been one of the powerful tools for biosensors and bioimaging. A wavelength-scale periodic structure coated with a thin metal film is called a plasmonic chip, and it can provide SPF. SPF of Cy5-streptavidin (Cy5-SA) was measured on a biotinylated plasmonic chip with a grating of 480 nm-pitch. The optimal structure of a plasmonic sensor-chip was designed for improving detection sensitivity. The silver film thickness dependence of the SPF intensity was measured under the irradiation of the top panel of a sensor chip. Furthermore, the dependence of the SPF intensity on the distance from the metal surface was also investigated. The optimal structure for the largest fluorescence enhancement factor was 150 nm-thick silver and 10 nm-thick SiO 2 layers due to the enhanced electric field (excitation field), the surface plasmon coupled emission (SPCE), and the interference effect with reflected light. The largest enhancement factor was found to be 170-fold. Furthermore, not only the largest fluorescence intensity but also stable lower background noise were found to be essential for higher-sensitive detection.
机译：表面等离子体激元场增强荧光（SPF）已成为生物传感器和生物成像的强大工具之一。涂有金属薄膜的波长级周期性结构称为等离子体芯片，它可以提供SPF。 Cy5-链霉亲和素（Cy5-SA）的SPF在具有480 nm间距光栅的生物素化等离子体芯片上测量。设计了等离子体传感器芯片的最佳结构以提高检测灵敏度。在传感器芯片的顶板的照射下测量SPF强度的银膜厚度依赖性。此外，还研究了SPF强度对距金属表面距离的依赖性。由于增强的电场（激发场），表面等离子体激元耦合发射（SPCE）以及对反射光的干扰效应，最大荧光增强因子的最佳结构是150 nm厚的银和10 nm厚的SiO 2层。 。发现最大的增强因子是170倍。此外，发现不仅最大的荧光强度而且稳定的较低背景噪声对于高灵敏度检测都是必不可少的。

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