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首页> 外文期刊>MBio >North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus
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North American Triple Reassortant and Eurasian H1N1 Swine Influenza Viruses Do Not Readily Reassort to Generate a 2009 Pandemic H1N1-Like Virus

机译：北美三重重排和欧亚H1N1猪流感病毒无法立即重排以产生2009年的H1N1流感大流行病毒

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The 2009 pandemic H1N1 virus (pH1N1) was derived through reassortment of North American triple reassortant and Eurasian avian-like swine influenza viruses (SIVs). To date, when, how and where the pH1N1 arose is not understood. To investigate viral reassortment, we coinfected cell cultures and a group of pigs with or without preexisting immunity with a Eurasian H1N1 virus, A/Swine/Spain/53207/2004 (SP04), and a North American triple reassortant H1N1 virus, A/Swine/Kansas/77778/2007 (KS07). The infected pigs were cohoused with one or two groups of contact animals to investigate viral transmission. In coinfected MDCK or PK15 continuous cell lines with KS07 and SP04 viruses, more than 20 different reassortant viruses were found. In pigs without or with preexisting immunity (immunized with commercial inactivated swine influenza vaccines) and coinfected with both viruses, six or seven reassortant viruses, as well as the parental viruses, were identified in bronchoalveolar lavage fluid samples from the lungs. Interestingly, only one or two viruses transmitted to and were detected in contact animals. No reassortant containing a gene constellation similar to that of pH1N1 virus was found in either coinfected cells or pigs, indicating that the reassortment event that resulted in the generation of this virus is a rare event that likely involved specific viral strains and/or a favorable, not-yet-understood environment. >IMPORTANCE The 2009 pandemic-like H1N1 virus could not be reproduced either in cell cultures or in pigs coinfected with North American triple reassortant H1N1 and Eurasian H1N1 swine influenza viruses. This finding suggests that the generation of the 2009 pandemic H1N1 virus by reassortment was a rare event that likely involved specific viral strains and unknown factors. Different reassortant viruses were detected in coinfected pigs with and without preexisting immunity, indicating that host immunity plays a relevant role in driving viral reassortment of influenza A virus.
机译：2009年大流行的H1N1病毒（pH1N1）是通过将北美三重分类病毒和欧亚禽样猪流感病毒（SIV）重新分类而衍生的。迄今为止，尚不清楚何时，如何以及在何处出现pH1N1。为了研究病毒的重组，我们将细胞培养物和一组具有或不具有预先存在的免疫力的猪分别感染了欧亚H1N1病毒A / Swine / Spain / 53207/2004（SP04）和北美三重重组H1N1病毒A / Swine /堪萨斯州/ 77778/2007（KS07）。将感染的猪与一组或两组接触动物一起饲养，以研究病毒传播。在带有KS07和SP04病毒的共感染MDCK或PK15连续细胞系中，发现了20多种不同的重配病毒。在没有或已有免疫力的猪（用商业灭活的猪流感疫苗免疫）并同时感染了两种病毒的猪中，在肺部支气管肺泡灌洗液样本中鉴定出六种或七种重配病毒以及亲本病毒。有趣的是，只有一种或两种病毒传播给接触动物并在接触动物中被检测到。在同时感染的细胞或猪中均未发现含有与pH1N1病毒相似的基因构象的重配子，这表明导致该病毒产生的重配事件是罕见事件，可能涉及特定的病毒株和/或有利的，尚未理解的环境。 >重要性 2009年的大流行性H1N1病毒无法在细胞培养物中或在感染了北美三重重配H1N1和欧亚H1N1猪流感病毒的猪中复制。这一发现表明，通过重组产生的2009大流行H1N1病毒是罕见的事件，可能涉及特定的病毒株和未知因素。在有和没有预先存在的免疫的共感染猪中检测到不同的重组病毒，表明宿主免疫在驱动甲型流感病毒的病毒重组中起着重要作用。

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