...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>MBio >Identification of Population Bottlenecks and Colonization Factors during Assembly of Bacterial Communities within the Zebrafish Intestine
【24h】

Identification of Population Bottlenecks and Colonization Factors during Assembly of Bacterial Communities within the Zebrafish Intestine

机译：斑马鱼肠内细菌群落组装过程中人口瓶颈和定居因素的确定。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ABSTRACT The zebrafish, Danio rerio , is a powerful model for studying bacterial colonization of the vertebrate intestine, but the genes required by commensal bacteria to colonize the zebrafish gut have not yet been interrogated on a genome-wide level. Here we apply a high-throughput transposon mutagenesis screen to Aeromonas veronii Hm21 and Vibrio sp. strain ZWU0020 during their colonization of the zebrafish intestine alone and in competition with each other, as well as in different colonization orders. We use these transposon-tagged libraries to track bacterial population sizes in different colonization regimes and to identify gene functions required during these processes. We show that intraspecific, but not interspecific, competition with a previously established bacterial population greatly reduces the ability of these two bacterial species to colonize. Further, using a simple binomial sampling model, we show that under conditions of interspecific competition, genes required for colonization cannot be identified because of the population bottleneck experienced by the second colonizer. When bacteria colonize the intestine alone or at the same time as the other species, we find shared suites of functional requirements for colonization by the two species, including a prominent role for chemotaxis and motility, regardless of the presence of another species. IMPORTANCE Zebrafish larvae, which are amenable to large-scale gnotobiotic studies, comprehensive sampling of their intestinal microbiota, and live imaging, are an excellent model for investigations of vertebrate intestinal colonization dynamics. We sought to develop a mutagenesis and tagging system in order to understand bacterial population dynamics and functional requirements during colonization of the larval zebrafish intestine. We explored changes in bacterial colonization dynamics and functional requirements when bacteria colonize a bacterium-free intestine, one previously colonized by their own species, or one colonized previously or simultaneously with a different species. This work provides a framework for rapid identification of colonization factors important under different colonization conditions. Furthermore, we demonstrate that when colonizing bacterial populations are very small, this approach is not accurate because random sampling of the input pool is sufficient to explain the distribution of inserts recovered from bacteria that colonized the intestines.
机译：摘要斑马鱼Danio rerio是研究脊椎动物肠道细菌定殖的强大模型，但是共生细菌定居斑马鱼肠道所需的基因尚未在全基因组水平上受到质疑。在这里，我们将高通量的转座子诱变筛选应用于veronii气单胞菌Hm21和弧菌sp。菌株ZWU0020在斑马鱼肠单独繁殖过程中以及彼此竞争时以及在不同的繁殖顺序中均如此。我们使用这些转座子标记的文库来跟踪不同殖民化制度中细菌种群的大小，并确定这些过程中所需的基因功能。我们显示与以前建立的细菌种群的种内竞争，而不是种间竞争，极大地降低了这两种细菌定殖的能力。此外，使用简单的二项式采样模型，我们表明在种间竞争的条件下，由于第二个定殖者遇到的种群瓶颈，无法确定定植所需的基因。当细菌单独或与其他物种同时在肠道内定殖时，我们发现这两个物种共同拥有一套殖民化的功能要求，包括对趋化性和运动性的重要作用，无论是否存在其他物种。重要事项斑马鱼幼虫适合进行大规模的生菌研究，肠道微生物群的全面采样以及实时成像，是研究脊椎动物肠道菌落动态的极佳模型。我们试图开发一种诱变和标记系统，以了解斑马鱼小肠肠道定殖过程中细菌种群动态和功能要求。当细菌在无细菌的小肠中定殖时，我们探索了细菌定殖动力学和功能要求的变化，其中一个是以前由自己的物种定殖的，另一个是先前或同时与其他物种定殖的。这项工作为快速确定在不同定植条件下重要的定植因子提供了框架。此外，我们证明了当定殖细菌种群很小时，这种方法是不准确的，因为输入池的随机采样足以解释从定植于肠道的细菌中回收的插入片段的分布。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号