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首页> 外文期刊>Frontiers in Environmental Science >Effect-directed analysis of Ah receptor-mediated potencies in microplastics deployed in a remote tropical marine environment
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Effect-directed analysis of Ah receptor-mediated potencies in microplastics deployed in a remote tropical marine environment

机译：针对在偏远热带海洋环境中部署的微塑料中Ah受体介导的效能的效果导向分析

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To facilitate the study of potential harmful compounds sorbed to microplastics, an effect-directed analysis using the DR CALUX? assay as screening tool for Aryl hydrocarbon receptor (AhR)-active compounds in extracts of marine deployed microplastics and chemical analysis of hydrophobic organic compounds (HOCs) was conducted. Pellets of three plastic polymers (low-density polyethylene (LDPE), high-density polyethylene (HDPE) and high-impact polystyrene (HIPS)) were deployed at Heron Island in the Great Barrier Reef, Australia, for up to 8 months. Detected AhR-mediated potencies (bio-TEQs) of extracted plastic pellets ranged from 15 pg/g to 100 pg/g. Contributions of target HOCs to the overall bioactivities were negligible. To identify the major contributors, remaining plastic pellets were used for fractionation with a gas chromatography (GC) fractionation platform featuring parallel mass spectrometric (MS) detection. The bioassay analysis showed two bioactive fractions of each polymer with bio-TEQs ranging from 5.7 pg/g to 14 pg/g. High resolution MS was used in order to identify bioactive compounds in the fractions. No AhR agonists could be identified in fractions of HDPE or LDPE. Via a multivariate statistical approach the polystyrene (PS) trimer 1e- Phenyl-4e-(1- phenylethyl)-tetralin was identified in fractions of HIPS and in fractions of the blank polymer of HIPS.
机译：为了便于研究吸附到微塑料上的潜在有害化合物，使用DR CALUX？进行了效果导向的分析。作为海洋部署的微塑料提取物中的芳烃受体（AhR）活性化合物的筛选工具，该方法进行了测定，并对疏水性有机化合物（HOC）进行了化学分析。三种塑料聚合物（低密度聚乙烯（LDPE），高密度聚乙烯（HDPE）和高抗冲聚苯乙烯（HIPS））的粒料在澳大利亚大堡礁的苍鹭岛部署了长达8个月的时间。检测到的提取的塑料颗粒的AhR介导的效能（bio-TEQ）为15 pg / g至100 pg / g。目标HOC对总体生物活性的贡献可忽略不计。为了确定主要的贡献者，将剩余的塑料颗粒用于具有平行质谱（MS）检测功能的气相色谱（GC）分馏平台进行分馏。生物测定分析显示每种聚合物的两个生物活性级分的生物TEQ范围为5.7 pg / g至14 pg / g。为了鉴定级分中的生物活性化合物，使用了高分辨率质谱。在HDPE或LDPE的馏分中未鉴定出任何AhR激动剂。通过多元统计方法，在HIPS的馏分和HIPS的空白聚合物的馏分中鉴定出了聚苯乙烯（PS）三聚体1e-苯基-4e-（1-苯乙基）-四氢萘。

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