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首页> 外文期刊>Medical Devices: Evidence and Research >Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model
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Inhibition of bacterial attachment and biofilm formation by a novel intravenous catheter material using an in vitro percutaneous catheter insertion model

机译：使用体外经皮导管插入模型通过新型静脉导管材料抑制细菌附着和生物膜形成

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Introduction: Despite sterile barrier precautions and vigorous skin antisepsis, percutaneous insertion of intravenous catheters has been shown to result in attachment to the catheter surface of bacteria residing in the deep structures of the skin. Such attachment poses the risk of biofilm formation and eventual catheter-related bloodstream infection (CRBSI). This study was undertaken to assess whether the non-coated surface treatment of a unique catheter material (ChronoFlex C? with BioGUARD?) could inhibit bacterial attachment and biofilm formation. Methods: A novel in vitro model and fluorescence microscopy were used to compare two intravascular catheter materials with respect to bacterial attachment and biofilm formation. The control material was a commonly used polyurethane. The study material was a unique copolymer, treated so as to remove surface additives, alter hydrophobicity and create surface micro-patterning. Outcomes were assessed using both a membrane potential indicator and a cell death reporter with appropriate fluorescent channels. Thus, bacterial cells attached to the catheter surface (living and dead) were imaged without mechanical disruption. Results: Both bacterial attachment and biofilm formation are significantly inhibited by the study catheter material. In fact, over 5 times more bacteria were able to attach and grow on the control polyurethane material than on the study material ( P =0.0020). Moreover, those few bacteria that were able to attach to the study material had a 1.5 times greater likelihood of dying. Conclusion: Using a novel in vitro percutaneous catheter insertion model, ChronoFlex C with BioGUARD is proven to significantly inhibit bacterial attachment and biofilm formation as compared with a commonly used polyurethane catheter material.
机译：简介：尽管采取了无菌屏障预防措施和强烈的皮肤防腐措施，但已证明经皮插入静脉内导管会导致细菌附着在导管表面，而细菌会残留在皮肤深层结构中。这种附着会带来生物膜形成和最终与导管相关的血流感染（CRBSI）的风险。进行这项研究以评估独特导管材料（带有BioGUARD？的ChronoFlex C？）的非涂层表面处理是否可以抑制细菌附着和生物膜形成。方法：采用新型体外模型和荧光显微镜对两种血管内导管材料在细菌附着和生物膜形成方面进行比较。对照材料是常用的聚氨酯。研究材料是一种独特的共聚物，经过处理可以去除表面添加剂，改变疏水性并创建表面微图案。使用膜电位指示剂和具有适当荧光通道的细胞死亡报告物评估结果。因此，附着在导管表面的细菌细胞（活着的和死亡的）被成像而没有机械破坏。结果：研究导管材料显着抑制细菌附着和生物膜形成。实际上，在对照聚氨酯材料上附着和生长的细菌比在研究材料上附着和生长的细菌多5倍（P = 0.0020）。此外，能够附着在研究材料上的少数细菌死亡的可能性是其1.5倍。结论：与新型聚氨酯导管材料相比，使用新型的体外经皮导管插入模型，带有BioGUARD的ChronoFlex C被证明可以显着抑制细菌附着和生物膜形成。

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