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首页> 外文期刊>Medical science monitor : >Response to UV and cisplatin-induced damage in human ovarian cancer cells overexpressing XPB and expressing or not a wild type p53
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Response to UV and cisplatin-induced damage in human ovarian cancer cells overexpressing XPB and expressing or not a wild type p53

机译：过度表达XPB和表达或不表达野生型p53的人卵巢癌细胞对UV和顺铂诱导的损伤的反应

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XPB is an essential component of the nucleotide excision repair and has been reported by immunoprecipitating studies to associate with p53. The role of the interaction between p53 and XPB in determining cell sensitivity to DNA damaging agents has still to be defined. We have evaluated the effects of XPB overexpression on UV and cisplatin (whose lesions are substrate for nucleotide excision repair pathway) sensitivities in a human ovarian cancer cell line, expressing or not a wild type like p53. XPB overexpression did not change significantly cellular sensitivities to cisplatin and UV treatments, independently of the presence of a wt p53. The overexpression of XPB protein did not result in significant changes in repair activity as demonstrated by the host cell reactivation assay performed in the different cell systems. Moreover the presence of high XPB levels did not change the transactivating activity of wt p53, measured as total cellular levels of p21waf1 after induction of DNA damage.
机译：XPB是核苷酸切除修复的重要组成部分，据免疫沉淀研究已报道XPB与p53相关。 p53和XPB之间的相互作用在确定细胞对DNA破坏剂的敏感性方面的作用仍有待确定。我们已经评估了XPB过表达对人类卵巢癌细胞系（表达或不表达野生型p53）中UV和顺铂（其损伤是核苷酸切除修复途径的底物）敏感性的影响。 XPB的过表达并没有明显改变细胞对顺铂和紫外线治疗的敏感性，而与wt p53的存在无关。 XPB蛋白的过表达并没有导致修复活性的显着变化，正如在不同细胞系统中进行的宿主细胞再激活测定所表明的。此外，高XPB水平的存在并没有改变wt p53的反式激活活性，以DNA损伤诱导后p21waf1的总细胞水平衡量。

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