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首页> 外文期刊>Medical science monitor : >Lubricating agents differ in their protection of cultured human epithelial cells against desiccation
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Lubricating agents differ in their protection of cultured human epithelial cells against desiccation

机译：润滑剂在保护培养的人上皮细胞抗干燥方面有所不同

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Background In dry eye, as a disease of the ocular surface, the instillation of artificial tears should compensate for the deficit in wetting and protect the mucosa against drying. Material and Method The desiccation protection of different pharmacological substances was tested using the conjunctival epithelial cell line Chang 1-5c-4 (series 1) and the corneal cell line 2.040 pRSV-T (series 2). On confluent cell growth the cultures were wetted for 20 min with various preservative-free preparations of artificial tears The cell cultures were exposed to a constant air flow for 0, 15, 30 and 45 minutes. Cells were incubated with the vital dye Alamar Blue and subsequently absorption of the oxidised form of the dye was measured using an ELISA-Reader. Results Cell survival rates in series 1 after 0, 15, 30, 45 min were (1.02;0.81;0.35;0.32) for Artelac EDO, (0.82;0.69; 0.63;0.54) for Vidisic EDO, (0.77;0.80;0.67;0.70) for Vidisic Fluid EDO, (0.76;0.70;0.36; 0.34) for Acuolens, (0.97;0.46;0.35;0.33) for Viscofresh, (0.88;0.85;0.37; 0.33) for Hyal Drops SDU, (0.71;0.44;0.34;0.33) for PBS and in series 2 (1.03;0.84;-0.21;-0.20) for Artelac EDO, (0.89;0.92;0.93;0.86) for Vidisic EDO, (0.96;0.88;0.85;0.85) for Vidisic Fluid EDO, (1.01;0.75;-0.02;-0.03) for Acuolens, (0.98;0.17;-0.22;-0.20) for Viscofresh, (0.97;0.83;0.03;-0.21) for Hyal Drops SDU and (0.96;0.26;-0.24;-0.21) for PBS. Vidisic Fluid EDO and Vidisic EDO showed a significantly better protective effect after a drying period of 30 and 45 min. Conclusions The protection capability of pharmacological substances against desiccation can be studied in a standardised cell culture system of human epithelial cell lines. Whether these in vitro results are conferrable to the efficacy of artificial tear drops in vivo has to be evaluated in clinical trials.
机译：背景技术在干眼症中，作为眼表疾病，滴入人工泪液应弥补润湿性不足并保护粘膜不干燥。材料和方法使用结膜上皮细胞系Chang 1-5c-4（系列1）和角膜细胞系2.040 pRSV-T（系列2）测试了不同药理物质的干燥保护作用。在汇合的细胞生长中，将培养物用各种不含防腐剂的人造眼泪湿润20分钟。将细胞培养物暴露于恒定的空气流中0、15、30和45分钟。将细胞与重要染料Alamar Blue一起孵育，随后使用ELISA-Reader测量该染料的氧化形式的吸收。结果Artelac EDO在0、15、30、45分钟后第1组的细胞存活率为（1.02; 0.81; 0.35; 0.32），对于Vidisic EDO为（0.82; 0.69; 0.63; 0.54），为（0.77; 0.80; 0.67;对于Vidisic Fluid EDO为0.70），对于Acuolens为（0.76; 0.70; 0.36; 0.34），对于Viscofresh为（0.97; 0.46; 0.35; 0.33），对于Hyal Drops SDU为（0.88; 0.85; 0.37; 0.33），（0.71; 0.44; PBS为0.34; 0.33），Artelac EDO为系列2（1.03; 0.84; -0.21; -0.20），Vidisic EDO为（0.89; 0.92; 0.93; 0.86），Vidisic Fluid为（0.96; 0.88; 0.85; 0.85） EDO，Acuolens为（1.01; 0.75; -0.02; -0.03），Viscofresh为（0.98; 0.17; -0.22; -0.20），Hyal Drops SDU为（0.97; 0.83; 0.03; -0.21）和（0.96; 0.26; -0.24; -0.21）（对于PBS）。在干燥30分钟和45分钟后，Vidisic Fluid EDO和Vidisic EDO表现出明显更好的保护效果。结论可以在人上皮细胞系的标准化细胞培养系统中研究药理物质对干燥的保护能力。这些体外结果是否可赋予体内人工泪液的功效，必须在临床试验中进行评估。

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