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首页> 外文期刊>Medical science monitor : >Changes in immunoreactivity of orexin-A-positive neurons after intravenous lipopolysaccharide injection
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Changes in immunoreactivity of orexin-A-positive neurons after intravenous lipopolysaccharide injection

机译：静脉注射脂多糖后食欲素A阳性神经元的免疫反应性变化

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Background Orexins are neuromediators that help regulate many different physiological processes; however, their role in regulating immune system functioning is unclear. This study sought to investigate the distribution of orexin in hypothalamic orexin-containing neurons during the first 6 hours after an intravenous injection of a lipopolysaccharide. Material and Method Orexin-containing neurons were detected by immunohistochemistry (avidin-biotin) on frontal sections from the brains of intact rats and rats after injections of either saline or lipopolysaccharide. Results Analysis of immunohistochemically stained sections (levels 26-32 according to Swanson's atlas) revealed that the main pool of orexin-containing neurons was located at brain levels 28 to 30. No changes were shown in the number of orexin-containing neurons in the hypothalamus of intact animals at different times of a day. Visualization of orexin-containing neurons in the lateral hypothalamus decreased only in 6 hours after an injection of lipopolysaccharide (500 microg/kg body weight) at levels 28, 29, and 30 of the rat brain; this might have been the result of changes in the balance of orexin synthesis and utilization. Conclusions These data suggest that orexin-containing neurons play a role in complex reactions in the brain to lipopolysaccharide application.
机译：背景食欲素是帮助调节许多不同生理过程的神经介质。然而，它们在调节免疫系统功能中的作用尚不清楚。这项研究旨在调查静脉注射脂多糖后的最初6个小时内下丘脑中含orexin的神经元中orexin的分布。材料和方法在免疫盐水或脂多糖注射后，通过免疫组织化学（亲和素-生物素）在完整大鼠和大鼠大脑的额叶上检测含有Orexin的神经元。结果免疫组织化学染色切片的分析（根据Swanson地图集的水平为26-32）显示，含食欲素的神经元的主要集合位于大脑水平28至30。下丘脑中含食欲素的神经元的数量没有变化。一天中不同时间的完整动物的数量。仅在注射大鼠脑中28、29和30水平的脂多糖（500微克/千克体重）后的6小时内，下丘脑外侧含食欲素的神经元的可视化才降低;这可能是食欲肽合成和利用平衡发生变化的结果。结论这些数据表明，含食欲素的神经元在大脑中对脂多糖应用的复杂反应中起作用。

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