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首页> 外文期刊>Medical science monitor : >Antibodies against Stachybotrys chartarum extract and its antigenic components, Stachyhemolysin and Stachyrase-A: a new clinical biomarker.
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Antibodies against Stachybotrys chartarum extract and its antigenic components, Stachyhemolysin and Stachyrase-A: a new clinical biomarker.

机译：针对金黄色葡萄球菌提取物及其抗原成分，水合溶血素和斯塔克切酶-A的抗体：一种新的临床生物标志物。

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BACKGROUND: IgG and IgE antibodies against Stachybotrys extract have been reported in allergic patients and residents of water-damaged buildings. Detection of these antibodies in blood was partially attributed to cross-reacting proteins from other fungi. There is a need for a specific method to detect antibodies against characteristic components of S. chartarum. MATERIAL/METHODS: We measured IgG and IgE antibodies against Stachybotrys hemolysin and proteinase-Stachyrase-A by ELISA and ELISA-inhibition techniques. RESULTS: Of 50 reference sera with IgE greater than 500 IU ml and positive against different mold extracts used in this study, significant elevation in IgG or IgE antibodies against S. chartarum extract was present in 25 and 21 specimens. Of these specimens 20 (80%) and 10 (40%) were positive for IgG anti-Stachybotrys hemolysin and anti-Stachyrase-A, while 8 out of 21 sera (38%) and 17 out of 21 (81%) specimens were positive for IgE anti-Stachybotrys hemolysin and anti-Stachyrase-A respectively. Inhibition studies using Stachybotrys hemolysin and Stachyrase-A at a concentration of 50 microg/ml prevented binding of anti-Stachybotrys to S. chartarum extract. CONCLUSIONS: Detection of IgG as well as IgE antibodies against Stachybotrys hemolysin and Stachyrase-A and inhibition of anti-Stachybotrys binding to Stachybotrys antigens indicate that Stachybotrys hemolysin and Stachyrase-A are two major antigenic components of S. chartarum extract, which can be used in antibody assays. Measurement of antibodies against these characteristic components of S. chartarum may be considered for demonstration of exposure and possibly allergy to the fungus.
机译：背景：在变态反应性患者和水毁建筑物的居民中，已报道了针对水生植物提取物的IgG和IgE抗体。血液中这些抗体的检测部分归因于来自其他真菌的交叉反应蛋白。需要一种检测针对沙门氏菌特征成分的抗体的特定方法。材料/方法：我们通过ELISA和ELISA抑制技术测量了针对水苏属溶血素的IgG和IgE抗体和蛋白酶-Stachyrase-A。结果：在本研究中使用的50份IgE大于500 IU ml的参考血清中，针对不同的霉菌提取物呈阳性，其中25份和21份标本中存在针对抗沙门氏菌提取物的IgG或IgE抗体。在这些标本中，有20个（80％）和10个（40％）的IgG抗史特博氏菌溶血素和抗Stachyrase-A呈阳性，而21个血清中的8个（38％）和21个血清中的17个（81％）均为阳性。分别对IgE抗志贺氏菌溶血素和抗Stachyrase-A呈阳性。使用Stachybotrys溶血素和Stachyrase-A的浓度为50 microg / ml的抑制研究可防止抗Stachybotrys与Chartarum提取物结合。结论：针对葡萄球菌溶血素和Stachyrase-A的IgG和IgE抗体的检测以及对Stachybotrys抗原结合的抗Stachybotrys的抑制作用表明，Stachybotrys溶血素和Stachyrase-A是沙特氏菌提取物的两个主要抗原成分，可以使用。在抗体检测中。可以考虑针对抗沙门氏菌的这些特征成分的抗体的测量，以证明对真菌的暴露以及可能的过敏。

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