...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Medical science monitor : >The effect of antitumor drugs on oxidative stress in B16 and S91 melanoma cells in vitro.
【24h】

The effect of antitumor drugs on oxidative stress in B16 and S91 melanoma cells in vitro.

机译：抗肿瘤药物对B16和S91黑色素瘤细胞体外氧化应激的影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: This study examined the effect of actinomycin-D (AMD), adriamycin (ADR), cisplatin (Cis-Pt), vincristine (VCR), cytosine arabinoside (Ara-C) and dacarbazine (DTIC) on the survival of B16 and S91 mouse melanoma cells in vitro, and on the concentration of thiobarbituric acid reactive substances (TBARS), the content of conjugated dienes (CD), and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). MATERIAL/METHODS: B16 and S91 mice melanoma cells were cultivated in culture medium. After the selected drugs had been added to the culture medium, the viability of the melanoma cells was tested using two methods: the cell count in a phase contrast microscope with inverted optics, and the MTT Test. RESULTS: The survival of melanoma cells after incubation with all cytostatic drugs was significantly lower than in the controls. The concentrations of CD and TBARS and the activity of antioxidant enzymes in melanoma cells after incubation with antitumor drugs were higher than in the controls. The highest concentration of CD and TBARS was shown after incubation with ADR. The highest activity of SOD was noticed after incubation with AMD. The highest activity of CAT was found after incubation with AMD in B16 cells and with Cis-Pt in S91 cells. The highest activity of GPx was found after incubation with Ara-C. CONCLUSIONS: The changes in antioxidant enzyme activity and the concentration of lipid peroxidation products confirm the participation of reactive oxygen species (ROS) in the cytotoxic action of antitumor drugs.
机译：背景：这项研究检查了放线菌素D（AMD），阿霉素（ADR），顺铂（Cis-Pt），长春新碱（VCR），胞嘧啶阿拉伯糖苷（Ara-C）和达卡巴嗪（DTIC）对B16和S91小鼠黑素瘤细胞的体外浓度，硫代巴比妥酸反应性物质（TBARS）的浓度，共轭二烯的含量（CD）以及超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。材料/方法：在培养基中培养B16和S91小鼠黑素瘤细胞。将选定的药物添加到培养基中后，使用两种方法测试黑素瘤细胞的活力：在具有倒置光学元件的相衬显微镜中的细胞计数和MTT测试。结果：所有细胞抑制药物孵育后，黑色素瘤细胞的存活率均明显低于对照组。与抗肿瘤药物孵育后，黑色素瘤细胞中CD和TBARS的浓度以及抗氧化酶的活性高于对照组。与ADR孵育后，CD和TBARS的浓度最高。与AMD一起孵育后，SOD活性最高。与AMD在B16细胞中和与Cis-Pt在S91细胞中孵育后，发现CAT的活性最高。与Ara-C孵育后，发现GPx的活性最高。结论：抗氧化酶活性的变化和脂质过氧化产物的浓度证实了活性氧（ROS）参与了抗肿瘤药物的细胞毒性作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号