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Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma

机译：毒力cagA基因幽门螺杆菌感染与喉鳞状细胞癌的关系

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Background The aim of the study was to evaluate the presence of cagA gene Helicobacter pylori in etiopathogenesis of initiation and development of larynx squamous cell carcinoma (LSCC) and its predictable role as a prognostic factor. Material and Methods The prospective, controlled study involved a series of 75 patients (65 male, 10 female, mean age 59.1 years, range 43 to 79 years) with larynx cancer. Samples of larynx cancerous tissue, each of 10–15 mg, were obtained from fresh tissues and were used for nucleic acid purification. DNA was extracted from 225 samples (larynx tumor – I (75), margin of tumor and normal tissue – II (75) and normal larynx tissue from opposite side to the tumor – III). All samples were subjected to H. pylori ureA detection by the PCR H. pylori diagnostic test. Samples that were positive for ureA H. pylori gene were evaluated for cagA H. pylori gene. Results Presence of H. pylori cagA gene was identified in 46,7% to 49,3% of 75 H. pylori ureA gene-positive larynx cancer depending of tissue location. There was a correlation of high incidence of positive cagA gene in larynx cancer tissue in supraglottic versus subglottic and glottic location. We observed a predominance of cagA gene in LSCC in patients with positive cervical lymph nodes and clinical stage T3 and T4. Conclusions H. pylori is present in larynx tissue and may be a possible carcinogen or co-carcinogen in LSCC development, but that must be addressed by future investigations. The presence of cagA gene in larynx cancer tissues significantly decreases survival rate and increases the disease recurrence possibilities.
机译：背景研究的目的是评估cagA基因幽门螺杆菌在喉鳞状细胞癌（LSCC）的发生和发展的病因中的存在及其作为预后因素的可预测作用。资料和方法这项前瞻性，对照研究涉及75例喉癌患者（男65例，女10例，平均年龄59.1岁，范围43至79岁）。从新鲜组织中获得喉癌组织样品，每份10–15 mg，用于核酸纯化。从225个样本中提取了DNA（喉肿瘤– I（75），肿瘤和正常组织的边缘– II（75）和正常的喉组织从肿瘤的另一侧– III）。通过PCR幽门螺杆菌诊断测试对所有样品进行幽门螺杆菌ureA检测。对ureA H. pylori基因呈阳性的样品评估cagA H. pylori基因。结果根据组织位置，在75例幽门螺杆菌ureA基因阳性的喉癌中，有46.7％至49,3％的患者发现了幽门螺杆菌cagA基因。声门上与声门下和声门位置的喉癌组织中阳性cagA基因的高发生率相关。我们观察到宫颈癌淋巴结阳性且临床分期为T3和T4的患者在LSCC中主要存在cagA基因。结论幽门螺杆菌存在于喉部组织中，可能是LSCC发展中的一种可能的致癌物或共致癌物，但必须通过今后的研究加以解决。喉癌组织中cagA基因的存在显着降低了存活率并增加了疾病复发的可能性。

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