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首页> 外文期刊>Medical Gas Research >Hydrogen sulphide-releasing aspirin enhances cell capabilities of anti-oxidative lesions and anti-inflammation
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Hydrogen sulphide-releasing aspirin enhances cell capabilities of anti-oxidative lesions and anti-inflammation

机译：释放硫化氢的阿司匹林可增强细胞抗氧化和抗发炎的能力

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Hydrogen sulphide (Hsub2/subS) has been considered as a toxic gas for a long time till new researches discovered the endogenous Hsub2/subS effects on physiological and pathological processes. In virtue of Hsub2/subS’s effects on cellular redox imbalance and aspirin’s good anticoagulation property, exogenous Hsub2/subS donors, such as Hsub2/subS-releasing aspirin (ACS14), have been explored to attenuate side effects of aspirin on gastrointestinal mucosal damage. However, existing researches mainly focus on the antithrombotic effects. Considering Hsub2/subS role in angiogenesis and vascular-protection progress, we herein focused on if ACS14 further has the ability to attenuate oxidative lesion and inflammation in human umbilical vein endothelial cells (HUVECs) and macrophages. In this study, we synthesized ACS14 by 5-(4-methoxyphenyl)-1,2-dithiole-3-thione and o-acetylsalicylic acid (aspirin), and the obtained compounds showed the ability to release Hsub2/subS. Our data illustrated that both aspirin and ACS14 had good cytocompatibility, and could support the proliferation of HUVECs. And, ACS14 was found to be able to promote 1.6 folds increase compared to aspirin. Hsub2/subS released from ACS14 was detected inside cells, wherein H2S fluorescence intensity increased twofold in 5 μM and 10 μM ACS14 groups than 1 μM group. Owing to reactive oxygen species inside cells being obviously decreased in ACS14 group, the apoptosis rate of HUVEC herein was reduced as low as 1.6% from 60% of blank group. Meanwhile, the tumour necrosis factor alpha release in macrophage was also declined by 15% in ACS14 groups than the others. Basically, the ACS14 we obtained had the cyto-protective and anti-inflammatory capabilities. Potential applications for vascular intima repair in atherosclerosis are further expected.
机译：直到新的研究发现内源性H 2 S对生理和病理过程的影响之前，硫化氢（H 2 S）一直被认为是有毒气体。借助于H 2 S对细胞氧化还原失衡的作用和阿司匹林的良好抗凝特性，外源H 2 S供体，例如H 2 S-已经研究了释放阿司匹林（ACS14）以减轻阿司匹林对胃肠道粘膜损伤的副作用。但是，现有的研究主要集中在抗血栓形成作用上。考虑到H 2 S在血管生成和血管保护进展中的作用，我们在此集中于ACS14是否进一步具有减弱人脐静脉内皮细胞（HUVEC）和巨噬细胞的氧化损伤和炎症的能力。本研究中，我们用5-（4-甲氧基苯基）-1，2-二硫代-3-硫酮和邻乙酰基水杨酸（阿司匹林）合成了ACS14，得到的化合物具有释放H 2 的能力。 sub> S。我们的数据表明，阿司匹林和ACS14都具有良好的细胞相容性，并且可以支持HUVEC的增殖。并且，发现ACS14与阿司匹林相比能够促进1.6倍的增加。在细胞内检测到从ACS14释放出的H 2 S，其中5μM和10μMACS14组的H2S荧光强度比1μM组增加了两倍。由于ACS14组细胞内的活性氧明显减少，因此HUVEC的凋亡率从空白组的60％降低到1.6％。同时，ACS14组的巨噬细胞中肿瘤坏死因子α的释放也下降了15％。基本上，我们获得的ACS14具有细胞保护和抗炎功能。进一步预期在动脉粥样硬化中血管内膜修复的潜在应用。

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