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首页> 外文期刊>Medicinal Chemistry >GV20 Penetrating GB7 on the Expression of Wnt1 in Brain Tissue of Rats with Intracerebral Hemorrhage
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GV20 Penetrating GB7 on the Expression of Wnt1 in Brain Tissue of Rats with Intracerebral Hemorrhage

机译：GV20穿透GB7对脑出血大鼠脑组织Wnt1表达的影响。

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Background: Intracerebral hemorrhage (ICH) is a life-threatening disease that confuses us for centuries. Acupuncture as a kind of Chinese traditional treatment has been empirically established and widely used in clinics in China, especially for stroke victims. Studies proved that GV20 penetrating GB7, a kind of needling manipulation, with which a needle is inserted into GV20 and then to GB7 under the scalp, is especially effective on the treatment of this disease. Wingless-type1 (Wnt1) is a key factor of Wnt/β-catenin signal pathway, which could promote regeneration and remodeling of neural function after ICH by affecting proliferation and differentiation of the neural stem cells (NSCs). The study on the expression of Wnt1 induced by GV20 penetrating GB7 may give out the theoretical principle of the treatment.Objective: The goal of this work is to observe the effect of GV20 penetrating GB7 needling on the protein/ mRNA expression of Wnt1 in brain tissue of ICH rats.Methods: In this experiment, we focused on observing the protein/mRNA expression level of Wnt1 by Western blot and real-time fluorescent quantitative PCR (RTFQ PCR) in the rats with ICH, which were treated by Dickkopfrelated protein 1 (DKK1), acupuncture or no intervention, comparing with the healthy rats without any treatment at fixed time points.Results: This study revealed that the expression of Wnt1 had the similar trend in all groups at each time point of corresponding treatment, which began to increase from the third day (3d), reached the peak on the seventh day (7d) then decreased gradually. The acupuncture treatment group showed a higher level of expression.Conclusion: The results show that GV20 penetrating GB7 has advantage on promoting the protein/mRNA expression of Wnt1, activates the intracellular Wnt pathway, and regulates the transcription of downstream target genes, affecting proliferation and differentiation of the neural stem cells (NSCs), thereby initiates endogenous repair mechanisms of the organism itself. This study provides a powerful scientific basis for acupuncture treatment on ICH.
机译：背景：脑出血（ICH）是一种威胁生命的疾病，使我们困惑了几个世纪。经验证明，针灸是一种中医传统疗法，并已在中国的诊所中广泛使用，尤其是中风患者。研究证明，穿透GV20的GB7是一种针刺操作，将针头插入GV20，然后在头皮下插入GB7尤其有效。 Wingless-type1（Wnt1）是Wnt /β-catenin信号通路的关键因素，通过影响神经干细胞（NSCs）的增殖和分化，可以促进ICH后神经功能的再生和重塑。对GV20穿透GB7诱导Wnt1表达的研究可能提供了治疗的理论原理。目的：本研究的目的是观察GV20穿透GB7针刺对脑组织中Wnt1蛋白/ mRNA表达的影响。方法：在本实验中，我们致力于通过Western blot和实时荧光定量PCR（RTFQ PCR）观察ICH大鼠的Wnt1蛋白/ mRNA表达水平，并用Dickkopf相关蛋白1（结果：这项研究表明，在相应治疗的每个时间点，所有组中Wnt1的表达都具有相似的趋势，并且开始增加。从第三天（3d）开始，在第七天（7d）达到峰值，然后逐渐下降。结论：GV20穿透GB7具有促进Wnt1蛋白/ mRNA表达，激活细胞内Wnt通路，调节下游靶基因转录，影响增殖和分化的作用。神经干细胞（NSCs）的分化，从而启动了生物体自身的内源性修复机制。该研究为针灸治疗ICH提供了有力的科学依据。

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