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Spatial and temporal variability of bacterial communities within a combined sewer system

机译:下水道系统中细菌群落的时空变化

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Abstract This study describes the temporal and spatial variability of bacterial communities within a combined sewer system in England. Sampling was conducted over 9 months in a sewer system with intensive monitoring of hydraulic conditions. The bacterial communities were characterized by 16S rRNA gene-targeted terminal restriction fragment length polymorphism analysis. These data were related to the hydraulic data as well as the sample type, location, and time. Temporal and spatial variation was observed between and within wastewater communities and biofilm communities. The bacterial communities in biofilm were distinctly different from the communities in wastewater and exhibited greater spatial variation, while the wastewater communities exhibited variability between different months of sampling. This study highlights the variation of bacterial communities between biofilm and wastewater, and has shown both spatial and temporal variations in bacterial communities in combined sewers. The temporal variation is of interest for in-sewer processes, for example, sewer odor generation, as field measurements for these processes are often carried out over short durations and may therefore not capture the influence of this temporal variation of the bacterial communities.
机译:摘要这项研究描述了英格兰下水道系统中细菌群落的时空变化。在下水道系统中进行了9个月的采样,对水力状况进行了严格监控。通过16S rRNA基因靶向终端限制性片段长度多态性分析来表征细菌群落。这些数据与水力数据以及样品类型,位置和时间有关。在废水群落和生物膜群落之间和内部观察到时间和空间变化。生物膜中的细菌群落与废水中的群落明显不同,并且表现出更大的空间变化,而废水群落在不同采样月份之间表现出变异性。这项研究突出了生物膜和废水之间细菌群落的变化,并显示了污水处理厂中细菌群落的时空变化。对于下水道过程,例如下水道气味的产生,时间变化是令人感兴趣的,因为这些过程的现场测量通常是在短时间内进行的,因此可能无法捕获细菌群落这种时间变化的影响。

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