• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>MicrobiologyOpen >Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005
【24h】

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

机译：食用蓝藻Arthrospira sp。的抗辐射性的分子研究。 PCC 8005

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AbstractThe aim of this work was to characterize in detail the response of Arthrospira to ionizing radiation, to better understand its radiation resistance capacity. Live cells of Arthrospira sp. PCC 8005 were irradiated with 60Co gamma rays. This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h−1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signaling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.
机译：摘要这项工作的目的是详细描述节肢动物对电离辐射的响应，以更好地了解其抗辐射能力。关节螺旋藻的活细胞。用 60 Co伽玛射线辐照PCC 8005。这项研究是第一项研究，表明节肢螺旋藻对伽马射线具有很高的耐受性，并且可以存活至少6400 Gy（剂量率为527 Gyh -1 ），从而鉴定了节肢螺旋藻。 PCC 8005作为抗辐射细菌。用3200或5000 Gy辐照后的生物化学，包括蛋白质组学和转录组学分析表明，光系统II量子产率下降，碳固定减少，色素，脂质和次级代谢产物合成减少。诱导了光敏和信号通路的转录，以及基于硫醇的抗氧化剂系统。转录组学确实显示了在RNA水平上ssDNA修复系统和移动遗传元件（MGE）的显着激活。令人惊讶的是，细胞没有诱导经典的抗氧化剂或DNA修复系统，例如超氧化物歧化酶（SOD）酶和RecA蛋白。节肢动物细胞缺乏过氧化氢酶基因和LexA阻遏物。辐照的节肢螺旋藻细胞确实能强烈诱导出一组保守的蛋白，这些蛋白在辐射抗性中的功能仍有待阐明，但它们是有希望探索的新颖途径。这项研究揭示了节肢动物的抗辐射性以及所涉及的分子系统，为进一步更好地利用它铺平了道路。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号