• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>MicrobiologyOpen >RNA-seq transcriptome analysis of a Pseudomonas strain with diversified catalytic properties growth under different culture medium
【24h】

RNA-seq transcriptome analysis of a Pseudomonas strain with diversified catalytic properties growth under different culture medium

机译：在不同培养基下具有多种催化特性的假单胞菌菌株的RNA-seq转录组分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Biocatalysis is an emerging strategy for the production of enantio-pure organic molecules. However, lacking of commercially available enzymes restricts the widespread application of biocatalysis. In this study, we report a Pseudomonas strain which exhibited versatile oxidation activity to synthesize chiral sulfoxides when growing under M9-toluene medium and reduction activity to synthesize chiral alcohols when on Luria-Bertani (LB) medium, respectively. Further comparative transcriptome analysis on samples from these two cultural conditions has identified 1038 differentially expressed genes (DEG). Gene Ontology (GO) enrichment and KEGG pathways analysis demonstrate significant changes in protein synthesis, energy metabolism, and biosynthesis of metabolites when cells cultured under different conditions. We have identified eight candidate enzymes from this bacterial which may have the potential to be used for synthesis of chiral alcohol and sulfoxide chemicals. This work provides insights into the mechanism of diversity in catalytic properties of this Pseudomonas strain growth with different cultural conditions, as well as candidate enzymes for further biocatalysis of enantiomerically pure molecules and pharmaceuticals.
机译：摘要生物催化是生产对映体纯有机分子的新兴策略。然而，缺乏可商购的酶限制了生物催化的广泛应用。在这项研究中，我们报告了一种假单胞菌菌株，分别在M9-甲苯培养基中生长时表现出多种氧化活性以合成手性亚砜，而在Luria-Bertani（LB）培养基中时则具有还原活性以合成手性醇。对来自这两种培养条件的样品的进一步比较转录组分析已鉴定出1038个差异表达基因（DEG）。基因本体论（GO）富集和KEGG通路分析表明，当细胞在不同条件下培养时，蛋白质合成，能量代谢和代谢物的生物合成发生显着变化。我们已经从该细菌中鉴定出八种候选酶，它们可能具有用于合成手性醇和亚砜化学物质的潜力。这项工作提供了对这种假单胞菌菌株在不同培养条件下生长的催化特性多样性机制的见解，以及用于进一步对映体纯分子和药物进行生物催化的候选酶。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号