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首页> 外文期刊>MicrobiologyOpen >The endochitinase ChiA Btt of Bacillus thuringiensis subsp. tenebrionis DSM-2803 and its potential use to control the phytopathogen Colletotrichum gloeosporioides
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The endochitinase ChiA Btt of Bacillus thuringiensis subsp. tenebrionis DSM-2803 and its potential use to control the phytopathogen Colletotrichum gloeosporioides

机译：苏云金芽孢杆菌亚种的内切几丁质酶ChiA Btt。 tenebrionis DSM-2803及其在控制植物病原菌炭疽菌中的潜在用途

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Abstract Bacillus thuringiensis subsp. tenebrionis DSM-2803 has been studied extensively and spore/crystal mixtures of this strain are used widely in commercial products to control coleopteran pests. The endochitinase chiA Btt gene of B. thuringiensis subsp. tenebrionis DSM-2803 was cloned and expressed in Escherichia coli . The recombinant 6x-histidine tagged protein (rChiA Btt, ~74 kDa), was purified by a HiTrap Ni affinity column. The K m of rChiA Btt was 0.847 ???? mol L ?￠????1 and its optimal activity occurred at pH 7 and ~40???°C. Most divalent cations reduced endochitinase activity but only Hg +2 abolished activity of the enzyme. We report for the first time the characterization of a chitinase synthesized by B. thuringiensis subsp. tenebrionis DSM-2803, and show that the purified rChiA74 Btt reduced the radial growth and increased the hyphal density of Colletotrichium gloeosporioides, the etiological agent of ?￠????anthracnose?￠???? in plants.
机译：苏云金芽孢杆菌亚种tenebrionis DSM-2803已被广泛研究，该菌株的孢子/晶体混合物已广泛用于商业产品中防治鞘翅目害虫。苏云金芽孢杆菌亚种的内切几丁质酶chiA Btt基因。在大肠杆菌中克隆并克隆了轮孢菌DSM-2803。重组6x-组氨酸标记的蛋白（rChiA Btt，〜74 kDa）通过HiTrap Ni亲和柱纯化。 rChiA Btt的K m为0.847 ???? mol L -1，其最佳活性发生在pH 7和约40℃。大多数二价阳离子降低了内切几丁质酶的活性，但只有Hg +2消除了该酶的活性。我们第一次报告由苏云金芽孢杆菌亚种合成的几丁质酶的表征。 tenebrionis DSM-2803，并显示纯化的rChiA74 Btt降低了炭疽病菌炭疽菌的炭疽病菌的径向生长并增加了菌丝密度，炭疽病菌是炭疽病的病原体。在植物中。

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