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Investigation of the microbial communities colonizing prepainted steel used for roofing and walling

机译：对用于屋顶和墙壁的预涂钢的微生物群落的调查

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Abstract Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design fouling prevention strategies. Here, next-generation sequencing and microbial community finger printing (T-RFLP) were used to study the community composition of microbes colonizing prepainted steel roofing materials at Burrawang, Australia and Kapar, Malaysia over a 52-week period. Community diversity was low and was dominated by Bacillus spp., cyanobacteria, actinobacteria, Cladosporium sp., Epicoccum nigrum , and Teratosphaeriaceae sp. Cultivation-based methods isolated approximately 20 different fungi and bacteria, some of which, such as E. nigrum and Cladosporium sp., were represented in the community sequence data. Fluorescence in situ hybridization imaging showed that fungi were the most dominant organisms present. Analysis of the sequence and T-RFLP data indicated that the microbial communities differed significantly between locations and changed significantly over time. The study demonstrates the utility of molecular ecology tools to identify and characterize microbial communities associated with the fouling of painted steel surfaces and ultimately can enable the targeted development of control strategies based on the dominant species responsible for fouling.
机译：摘要常用于屋面应用的预涂钢的微生物菌落会影响其美观性，耐用性和功能性。了解相关生物及其发生定植的机制将提供有价值的信息，这些信息随后可用于设计防污策略。在这里，下一代测序和微生物群落指纹图谱（T-RFLP）用于研究在52周的时间内在澳大利亚Burrawang和马来西亚Kapar上定植的钢制屋面材料中微生物的群落组成。群落多样性低，主要由芽孢杆菌属，蓝细菌，放线菌，枝孢属，黑附球菌和毛状球菌属所主导。基于耕种的方法分离了大约20种不同的真菌和细菌，其中的一些，例如黑曲霉和克拉多孢菌（Cladosporium sp。），在群落序列数据中得到了体现。荧光原位杂交成像显示真菌是目前最主要的生物。对序列和T-RFLP数据的分析表明，不同位置之间的微生物群落差异显着，并且随时间变化显着。这项研究证明了分子生态学工具可用于识别和表征与喷漆钢表面结垢有关的微生物群落，并最终能够基于负责结垢的优势物种有针对性地开发控制策略。

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