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Isolation of Bdellovibrio sp. from soil samples in Mexico and their potential applications in control of pathogens

机译:Bdellovibrio sp。的分离。墨西哥土壤样品中的提取及其在病原体控制中的潜在应用

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Abstract In this study, two strains of Bdellovibrio were isolated from soil samples using the culture-dependent technique and two members of the family Enterobacteriaceae ( Klebsiella sp. and Salmonella sp.) as prey. The Bdellovibrio strains were bacteriolytic, plaque-forming, and highly motile gram-negative bacteria. We identified and confirmed the Bdellovibrio strains using microscopy, PCR amplification, and sequencing of the 16S rRNA gene. They were observed to be different strains based on hit locus and prey range analyses. Here, the first report on Bdellovibrio strains isolated from soil in Mexico corroborates earlier report indicating that populations of Bdellovibrio found in soil are heterogeneous thereby the need to identify the various strains.
机译:摘要在本研究中,使用培养依赖性技术从土壤样品中分离出两株Bdellovibrio菌株,并以肠杆菌科的两个成员(Klebsiella sp。和Salmonella sp。)作为猎物。 Bdellovibrio菌株是细菌分解,噬菌斑形成和能动性革兰氏阴性菌。我们使用显微镜,PCR扩增和16S rRNA基因测序鉴定并确认了Bdellovibrio菌株。根据命中位点和猎物范围分析,发现它们是不同的菌株。在这里,关于墨西哥从土壤中分离出的Bdellovibrio菌株的第一份报告证实了较早的报道,该报告表明在土壤中发现的Bdellovibrio种群是异质的,因此需要鉴定各种菌株。

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