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首页> 外文期刊>International Journal of Vascular Medicine >Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results
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Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results

机译：本构型一氧化氮合酶亚型在静脉曲张壁中的表达初步结果

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There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpairedt-test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works.
机译：在文献中关于原发性静脉曲张的结构变化存在矛盾的发现。 NO（有效的血管扩张剂）是由一氧化氮合酶（NOS）合成的。从3种已知的NOS亚型中，两种是组成型：eNOS（内皮型NOS）和nNOS（神经型NOS）。使用抗eNOS和nNOS的兔多克隆抗体，通过标准光学显微镜和免疫组织化学技术处理10个静脉曲张和10个对照静脉样品。半定量评估抗体表达，并通过2D图像分析在形态上证明。通过颜色分析和颜色数字减法确定NOS同工型表达的总面积。结果显示，与对照组相比，曲张静脉的中膜中两种NOS亚型的表达均不连续且明显较低。为了进行统计分析，使用了不成对检验。我们的研究结果推测静脉曲张壁NO含量较低，推测静脉曲张扩张是由于其他机制引起的，并且与先前发表的类似研究结果相矛盾。

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