Unexpected Effects of Wnt-1 on the Differentiation and Adhesion of Myogenic Cells

Mindy George-Weinstein; Jeffrey Dare; Jacquelyn Gerhart; Anthony M.C. Brown; Ann Redfield; Heather Brown; Karen A. Knudsen

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Unexpected Effects of Wnt-1 on the Differentiation and Adhesion of Myogenic Cells

机译：Wnt-1对成肌细胞分化和粘附的意外影响

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Wnt proteins are critical for the development of a wide range of organisms. Wnt-1 is expressed by the neural tube and promotes skeletal myogenesis in cultures of intact somites. In other cell types, Wnt-1 enhances cell-cell adhesion mediated by cadherin/catenin complexes. The effect of Wnt-1 on myogenesis was examined in cultures of dissociated epiblast, segmental plate and somite tissues. Wnt-1 was provided by Rat-2 cells expressing Wnt-1 cDNA. Wnt-1 did not promote myogenesis in prestreak epiblast cultures. In primitive streak stage epiblast cultures, Wnt-1 inhibited myogenesis and the shift from E- to N-cadherin. Both the control and Wnt-1 expressing Rat-2 cells inhibited differentiation of somite and segmental plate cells; however, the inhibition was less for somite cells cultured with Wnt-1. The Rat-2 cells have myogenic potential in that they express desmin, M-cadherin, NCAM, MyoD and MEF-2, but they do not differentiate. They respond to Wnt-1 with an increase in b -catenin, a decrease in cell-cell adhesion and a downregulation of NCAM. The effects of Wnt-1 on adhesion and differentiation depend on intrinsic cell properties that vary with the stage of development.

机译：Wnt蛋白对于广泛的生物体的发展至关重要。 Wnt-1由神经管表达，并在完整体节的培养中促进骨骼肌发生。在其他细胞类型中，Wnt-1增强钙粘蛋白/连环蛋白复合物介导的细胞间粘附。在解离的上皮细胞，节段性板块和节肢组织的培养物中检查了Wnt-1对肌发生的作用。 Wnt-1由表达Wnt-1 cDNA的Rat-2细胞提供。 Wnt-1不会在前条纹前胚层培养物中促进肌发生。在原始条纹阶段的成骨细胞培养中，Wnt-1抑制肌发生和从E-向N-钙粘蛋白的转移。表达对照和表达Wnt-1的Rat-2细胞均抑制体细胞和节段性板细胞的分化。然而，对用Wnt-1培养的体细胞的抑制作用较小。 Rat-2细胞具有成肌潜力，因为它们表达结蛋白，M-钙黏着蛋白，NCAM，MyoD和MEF-2，但它们不分化。他们对Wnt-1的反应是b-连环蛋白的增加，细胞间粘附的减少和NCAM的下调。 Wnt-1对粘附和分化的影响取决于固有的细胞特性，该特性随发育阶段而变化。

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《European Journal of Translational Myology》 |1998年第4期|共12页
作者
Mindy George-Weinstein; Jeffrey Dare; Jacquelyn Gerhart; Anthony M.C. Brown; Ann Redfield; Heather Brown; Karen A. Knudsen;
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中图分类肌肉疾病及损伤;
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机译：MCAM有助于在成肌和软骨分化中建立细胞自主极性MCAM有助于在成肌和软骨分化中建立细胞自主极性MCAM有助于在成肌和软骨分化中建立细胞自主极性
4. Graphene oxide-stimulated myogenic differentiation of C2C12 cells on PLGA/RGD peptide nanofiber matrices [C] . Y. C. Shin, J. H. Lee, M. J. Kim, Conference on nano-bio sensing, imaging, and spectroscopy . 2015

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6. Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing beta-catenin binding to the cell adhesion protein cadherin [O] . 1994

机译：Wnt-1通过稳定β-catenin与细胞粘附蛋白钙黏着蛋白的结合来调节哺乳动物细胞中的细胞粘附
7. "Trans-differentiation" from epidermal to mesenchymal/myogenic phenotype is associated with a drastic change in cell-cell and cell- matrix adhesion molecules [O] . 1993

机译：从表皮到间充质/成肌表型的“转分化”与细胞-细胞和细胞-基质粘附分子的剧烈变化有关

Unexpected Effects of Wnt-1 on the Differentiation and Adhesion of Myogenic Cells

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