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首页> 外文期刊>Modern Chemistry & Applications >Non-Edible Vernonia galamensis Oil and Mixed Bacterial Cultures for the Production of Polyhydroxyalkanoates
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Non-Edible Vernonia galamensis Oil and Mixed Bacterial Cultures for the Production of Polyhydroxyalkanoates

机译：不可食用的食用菌Vernonia galamensis油和混合细菌培养物，用于生产聚羟基链烷酸酯

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Since the oil crisis of the 1970s much attempt has been made, albeit with varying degrees of success, to source the ideal substrate and bacteria for the production of PHA. The non-edible, naturally epoxidized seed oil from Vernonia galamensis and mixed cultures consisting of Alcaligenes latus (ATCC 29712), Cupriavidus necator (ATCC 17699), Escherichia coli (DH5α) and Pseudomonas oleovorans (ATCC 29347), were evaluated for PHA production under batch and fed-batch fermentations. PHA production, optimized by the mixed culture of E. coli and C. necator, was 0.4-19% (%wt/wt, cdw) for batch and fed-batch fermentations. Analyses of PHA by Matrix Assisted Laser Desorption Ionization- Time of Flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography Mass Spectrometry (GC/MS) identified the 3-hydroxybutyrate (3HB) monomeric unit. The PHA ester bond stretching vibration (C=O), was confirmed at absorption 1740.66 cm-1, using Fourier Transform Infrared Spectroscopy (FTIR). Gel Permeation Chromatography (GPC) indicated peak molecular weights between 3.8×103-1.12×106 Da with melting points (Tm), 60-90°C. The data further illustrates that inedible oils could be the ideal carbon source for the production of PHA.
机译：自1970年代的石油危机以来，尽管取得了不同程度的成功，但仍进行了许多尝试，以寻找理想的底物和细菌来生产PHA。评估了来自Vernonia galamensis的不可食用的天然环氧化种子油以及由Alcaligenes latus（ATCC 29712），Cupriavidus necator（ATCC 17699），Escherichia coli（DH5α）和油假单胞菌（Pseudomonas oleovorans）（ATCC 29347）组成的混合培养物的PHA生产分批和补料分批发酵。通过分批发酵和补料分批发酵，通过大肠杆菌和弯曲杆菌的混合培养优化的PHA产量为0.4-19％（％wt / wt，cdw）。通过基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF MS）和气相色谱质谱（GC / MS）对PHA进行分析，确定了3-羟基丁酸酯（3HB）单体单元。使用傅立叶变换红外光谱法（FTIR）在吸收1740.66 cm-1处确认了PHA酯键的拉伸振动（C = O）。凝胶渗透色谱法（GPC）显示峰分子量在3.8×103-1.12×106 Da之间，熔点（Tm）为60-90°C。数据进一步表明，食用油可能是生产PHA的理想碳源。

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