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首页> 外文期刊>Memorias do Instituto Oswaldo Cruz >Effect of Angiotensin II and Losartan on the Phagocytic Activity of Peritoneal Macrophages from Balb/C Mice
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Effect of Angiotensin II and Losartan on the Phagocytic Activity of Peritoneal Macrophages from Balb/C Mice

机译：血管紧张素Ⅱ和氯沙坦对Balb / C小鼠腹膜巨噬细胞吞噬活性的影响

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摘要

Angiotensin II (AII), a product of rennin-angiotensin system, exerts an important role on the function of immune system cells. In this study, the effect of AII on the phagocytic activity of mouse peritoneal macrophages was assessed. Mice peritoneal macrophages were cultured for 48 h and the influence of different concentrations of AII (10-14 to 10-7 M) and/or losartan, 10-16 to 10-6 M), an AT1 angiotensin receptor antagonist, on phagocytic activity and superoxide anion production was determined. Dimethylthiazoldiphenyltetrazolium bromide reduction and the nucleic acid content were used to assess the cytotoxicity of losartan. A stimulatory effect on phagocytic activity (P < 0.05) was observed with 10-13 M and 10-12 M AII concentrations. The addition of losartan (up to10-14 M) to the cell cultures blocked (P < 0.001) the phagocytosis indicating the involvement of AT1 receptors. In contrast, superoxide anion production was not affected by AII or losartan. The existence of AT1 and AT2 receptors in peritoneal macrophages was demonstrated by immunofluorescence microscopy. These results support the hypothesis that AII receptors can modulate murine macrophage activity and phagocytosis, and suggest that AII may have a therapeutic role as an immunomodulatory agent in modifying the host resistance to infection.
机译：血管紧张素-血管紧张素系统的产物血管紧张素II（AII）在免疫系统细胞的功能中起重要作用。在这项研究中，评估了AII对小鼠腹膜巨噬细胞吞噬活性的影响。小鼠腹膜巨噬细胞培养48小时，不同浓度的AII（10-14至10-7 M）和/或氯沙坦（10-16至10-6 M）（AT1血管紧张素受体拮抗剂）对吞噬活性的影响并确定了超氧阴离子的产生。溴化二甲基噻唑烷二苯基四唑鎓的还原和核酸含量用于评估氯沙坦的细胞毒性。在10-13 M和10-12 M AII浓度下，观察到了对吞噬活性的刺激作用（P <0.05）。向细胞培养物中添加氯沙坦（最多10-14 M）可阻止（P <0.001）吞噬作用，表明AT1受体参与。相反，超氧阴离子的产生不受AII或氯沙坦的影响。免疫荧光显微镜证实腹膜巨噬细胞中AT1和AT2受体的存在。这些结果支持AII受体可以调节鼠巨噬细胞活性和吞噬作用的假说，并且表明AII可以作为免疫调节剂在改变宿主对感染的抵抗力中具有治疗作用。

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