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首页> 外文期刊>Molecular biology of the cell >Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.
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Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

机译：KAR2和SEC63之间的遗传相互作用，编码内质网中DnaK和DnaJ的真核同源物。

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摘要

KAR2 encodes the yeast homologue of mammalian BiP, the endoplasmic reticulum (ER) resident member of the HSP70 family. Kar2p has been shown to be required for the translocation of proteins across the ER membrane as well as nuclear fusion. Sec63, an ER integral membrane protein that shares homology with the Escherichia coli DnaJ protein, is also required for translocation. In this paper we describe several specific genetic interactions between these two proteins, Kar2p and Sec63p. First, temperature-sensitive mutations in KAR2 and SEC63 form synthetic lethal combinations. Second, dominant mutations in KAR2 are allele-specific suppressors for the temperature-sensitive growth and translocation defect of sec63-1. Third, the sec63-1, unlike other translocation defective mutations, results in the induction of KAR2 mRNA levels. Taken together, these genetic interactions suggest that Kar2p and Sec63p interact in vivo in a manner similar to that of the E. coli HSP70, DnaK, and DnaJ. We propose that the interaction between these two proteins is critical to their function in protein translocation.
机译：KAR2编码哺乳动物BiP（HSP70家族的内质网（ER）驻留成员）的酵母同源物。已经证明，Kar2p是蛋白质跨ER膜转运以及核融合所必需的。 Sec63是一种ER整体膜蛋白，与大肠杆菌DnaJ蛋白具有同源性，也需要转运。在本文中，我们描述了这两种蛋白质Kar2p和Sec63p之间的几种特定的遗传相互作用。首先，KAR2和SEC63中的温度敏感突变形成合成的致死组合。其次，KAR2中的显性突变是sec63-1对温度敏感的生长和易位缺陷的等位基因特异性抑制剂。第三，与其他易位缺陷突变不同，sec63-1导致KAR2 mRNA水平的诱导。两者合计，这些遗传相互作用表明Kar2p和Sec63p在体内以类似于大肠杆菌HSP70，DnaK和DnaJ的方式相互作用。我们建议这两种蛋白质之间的相互作用对它们在蛋白质转运中的功能至关重要。

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