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首页> 外文期刊>Molecular biology of the cell >Induction of NF-kappa B-like activity by platelet-derived growth factor in mouse fibroblasts.
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Induction of NF-kappa B-like activity by platelet-derived growth factor in mouse fibroblasts.

机译：血小板衍生的生长因子在小鼠成纤维细胞中诱导NF-κB样活性。

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Nuclear factor kappa B (NF-kappa B) modulates the expression of numerous genes via interaction with a specific DNA sequence termed the kappa B site. Its activity is modulated by a cytosolic inhibitor protein termed I kappa B, and its activation occurs in response to a variety of agents in a variety of cell types, most notably B and T lymphocytes. Data presented here show that an activity (designated complex I) that binds specifically to the kappa B site is induced in density-arrested Balb/c-3T3 mouse fibroblasts by platelet-derived growth factor (PDGF), a potent mitogen for these cells. Increased levels of complex I, as evaluated by electrophoretic mobility shift assays of nuclear extracts, were observed in cells treated for 1-4 h (but not 15 min) with the BB isoform of PDGF. 12-O-tetradecanoylphorbol 13-acetate (TPA) and the AA isoform of PDGF also stimulated this response and both isoforms, but not TPA, were effective in cells depleted of protein kinase C. Complex I most likely is authentic NF-kappa B, a p50-p65 heterodimer, or a closely related factor because it exhibited properties characteristic of those previously described for NF-kappa B including inducibility by deoxycholate and cycloheximide and sensitivity to I kappa B. A second kappa B binding activity (complex II), which apparently contained p50 homodimers, displayed limited induction by PDGF, whereas a third complex (complex III) migrated faster than but behaved similarly to complex I. These studies suggest that NF-kappa B or an NF-kappa B-like factor may participate in the expression of PDGF-inducible genes.
机译：核因子κB（NF-κB）通过与称为κB位点的特定DNA序列的相互作用来调节众多基因的表达。它的活性受到称为IκB的胞质抑制剂蛋白的调节，并且其激活是响应多种细胞类型中最多种B和T淋巴细胞中的多种因子而发生的。此处显示的数据表明，血小板衍生的生长因子（PDGF）（这些细胞的有效促分裂原）可在密度停滞的Balb / c-3T3小鼠成纤维细胞中诱导特异性结合κB位点的活性（指定的复合物I）。通过核提取物的电泳迁移率迁移分析评估，在用PDGF的BB亚型处理1-4小时（但不是15分钟）的细胞中观察到复合物I的水平升高。 12-O-十四烷酰佛波醇13-乙酸盐（TPA）和PDGF的AA亚型也刺激了这种反应，并且这两种亚型（而非TPA）在耗尽蛋白激酶C的细胞中均有效。复合物I最有可能是真实的NF-κB， p50-p65异二聚体或密切相关的因子，因为它表现出先前描述的NF-κB的特性，包括脱氧胆酸盐和环己酰亚胺的诱导性以及对IκB的敏感性。第二kappa B的结合活性（复合物II）显然包含p50同二聚体，显示受PDGF诱导的作用有限，而第三种复合物（复合物III）的迁移速度快于复合物I，但其行为与复合物I相似。 PDGF诱导基因的表达。

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