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首页> 外文期刊>Molecular biology of the cell >Expression of NG2 proteoglycan causes retention of type VI collagen on the cell surface.
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Expression of NG2 proteoglycan causes retention of type VI collagen on the cell surface.

机译：NG2蛋白聚糖的表达导致VI型胶原在细胞表面保留。

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摘要

NG2 is a membrane-associated chondroitin sulfate proteoglycan with a core protein of 300 kD. Previously it was shown immunochemically that the core protein of NG2 can bind type VI collagen (Stallcup, W., Dahlin, K., and P. Healy. 1990. J. Cell Biol. 111:3177-3188). We have extended our studies on the interaction of NG2 and type VI collagen by transfecting cells with the full-length rat NG2 cDNA. B28 rat neural cells and U251MG human glioma cells used for transfection do not synthesize NG2. Both cell lines secrete type VI collagen into tissue culture medium but do not anchor it at the cell surface. Upon transfection of these cells with the NG2 cDNA, NG2 was correctly localized to the cell surface. Furthermore, type VI collagen could now be detected on the surface of NG2-positive cells in a pattern that coincided with that of NG2. This ability of NG2 to anchor type VI collagen to the cell surface could be abolished by incubating the cells in the presence of anti-NG2 monoclonal antibodies. These findings indicate that NG2 functions as a cell surface receptor for type VI collagen and may play a role in modulating the assembly of pericellular matrix.
机译：NG2是膜相关的硫酸软骨素蛋白聚糖，核心蛋白为300 kD。以前，免疫化学表明NG2的核心蛋白可以结合VI型胶原（Stallcup，W.，Dahlin，K。和P.Healy.1990.J.Cell Biol.111：3177-3188）。我们已经通过用全长大鼠NG2 cDNA转染细胞扩展了NG2和VI型胶原相互作用的研究。用于转染的B28大鼠神经细胞和U251MG人类神经胶质瘤细胞不合成NG2。两种细胞系都将VI型胶原分泌到组织培养基中，但不将其锚定在细胞表面。用NG2 cDNA转染这些细胞后，NG2正确定位在细胞表面。此外，现在可以在NG2阳性细胞的表面上检测到与NG2一致的VI型胶原蛋白。通过在抗NG2单克隆抗体的存在下孵育细胞，可以消除NG2将VI型胶原锚定到细胞表面的这种能力。这些发现表明，NG2充当VI型胶原的细胞表面受体，并可能在调节细胞周围基质的组装中发挥作用。

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