...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular biology of the cell >Vesicle Docking to the Spindle Pole Body Is Necessary to Recruit the Exocyst During Membrane Formation in Saccharomyces cerevisiae
【24h】

Vesicle Docking to the Spindle Pole Body Is Necessary to Recruit the Exocyst During Membrane Formation in Saccharomyces cerevisiae

机译：在酿酒酵母的膜形成过程中，必须将囊泡对接至主轴杆体才能吸收囊泡。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During meiosis II in Saccharomyces cerevisiae , the cytoplasmic face of the spindle pole body, referred to as the meiosis II outer plaque (MOP), is modified in both composition and structure to become the initiation site for de novo formation of a membrane called the prospore membrane. The MOP serves as a docking complex for precursor vesicles that are targeted to its surface. Using fluorescence resonance energy transfer analysis, the orientation of coiled-coil proteins within the MOP has been determined. The N-termini of two proteins, Mpc54p and Spo21p, were oriented toward the outer surface of the structure. Mutations in the N-terminus of Mpc54p resulted in a unique phenotype: precursor vesicles loosely tethered to the MOP but did not contact its surface. Thus, these mpc54 mutants separate the steps of vesicle association and docking. Using these mpc54 mutants, we determined that recruitment of the Rab GTPase Sec4p, as well as the exocyst components Sec3p and Sec8p, to the precursor vesicles requires vesicle docking to the MOP. This suggests that the MOP promotes membrane formation both by localization of precursor vesicles to a particular site and by recruitment of a second tethering complex, the exocyst, that stimulates downstream events of fusion.
机译：在酿酒酵母（Saccharomyces cerevisiae）的减数分裂II期间，纺锤极体的胞质面被称为减数分裂II外斑（MOP），其组成和结构均被修饰，成为从头形成膜的新位点，称为原生孢子。膜。 MOP用作针对其表面的前体囊泡的对接复合体。使用荧光共振能量转移分析，已确定了MOP中卷曲螺旋蛋白的方向。 Mpc54p和Spo21p这两种蛋白质的N末端朝向结构的外表面。 Mpc54p N末端的突变产生独特的表型：前体囊泡松散地束缚在MOP上，但未接触其表面。因此，这些mpc54突变体分离了囊泡缔合和对接的步骤。使用这些mpc54突变体，我们确定Rab GTPase Sec4p以及囊胚成分Sec3p和Sec8p募集到前体囊泡需要将囊泡对接至MOP。这表明，MOP既可以通过将前体囊泡定位到特定部位，也可以通过募集刺激下游融合的第二种系留复合物（囊泡）来促进膜形成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号