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首页> 外文期刊>Modern Pathology >Identification of microRNA expression profile related to lymph node status in women with early-stage grade 1-2 endometrial cancer
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Identification of microRNA expression profile related to lymph node status in women with early-stage grade 1-2 endometrial cancer

机译：早期1-2级子宫内膜癌女性与淋巴结状态相关的microRNA表达谱的鉴定

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Conventional methods used for histologic classification and grading of endometrial cancer (EC) are not sufficient to predict lymph node metastases. microRNA signatures have recently been related to EC pathologic characteristics or prognosis. The aim of this study was to evaluate whether microRNA profiles of grade 1鈥? endometrioid adenocarcinomas can be related to nodal status and used as a tool to adapt surgical staging in early-stage EC. microRNA expression was assessed in nine formalin-fixed paraffin-embedded (FFPE) EC primary tumors with positive lymph node and in 27 FFPE EC primary tumors with negative lymph node, matched for grade, stage, and lymphovascular space involvement status. A microarray analysis showed that there was more than a twofold significant difference in the expression of 12 microRNAs between the two groups. A quantitative reverse transcriptase鈥揚CR assay was used to confirm these results: the expression levels of five microRNAs (microRNA-34c-5p, -375, -184, -34c-3p, and -34b-5p) were significantly lower in the EC primary tumor with positive lymph node compared with those with negative lymph node. A minimal P-value approach revealed that women with a microRNA-375-fold change 0.30 (n=1; 4.8%), P=0.001. Furthermore, women with a microRNA 184-fold change 0.30 (n=3; 11.5%), P=0.006. This is the first study investigating the relative expression of mature microRNA genes in early-stage grade 1鈥? EC primary tumors according to the nodal status. This microRNA expression profile provides a potential basis for further study of the microRNA function in EC and could be used as a diagnostic tool for nodal status.
机译：用于子宫内膜癌（EC）的组织学分类和分级的常规方法不足以预测淋巴结转移。 microRNA签名最近与EC病理特征或预后有关。这项研究的目的是评估是否1级的microRNA谱？子宫内膜样腺癌可能与淋巴结状态有关，并用作适应早期EC手术分期的工具。在9例淋巴结阳性的福尔马林固定石蜡包埋（FFPE）EC原发性肿瘤和27例淋巴结阴性的FFPE EC原发性肿瘤中评估了microRNA的表达，以分级，分期和淋巴管空间受累状态相匹配。芯片分析表明，两组之间的12种microRNA的表达之间存在超过两倍的显着差异。使用定量逆转录酶'扬CR法来证实这些结果：五个microRNA（microRNA-34c-5p，-375，-184，-34c-3p和-34b-5p）的表达水平显着降低。淋巴结阳性的EC原发性肿瘤与阴性淋巴结的EC原发性肿瘤相比。最小P值方法显示，microRNA-375倍变化的女性为0.30（n = 1; 4.8％），P = 0.001。此外，microRNA 184倍变化的女性为0.30（n = 3; 11.5％），P = 0.006。这是第一个研究成熟的microRNA基因在早期1'？级相对表达的研究。 EC原发肿瘤根据淋巴结状态而定。该microRNA表达谱为进一步研究EC中的microRNA功能提供了潜在的基础，并可作为节点状态的诊断工具。

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