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首页> 外文期刊>Modern Pathology >Transformation-specific matrix metalloproteinases, MMP-7 and MMP-13, are present in epithelial cells of keratoacanthomas
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Transformation-specific matrix metalloproteinases, MMP-7 and MMP-13, are present in epithelial cells of keratoacanthomas

机译：角化棘皮瘤的上皮细胞中存在转化特异性基质金属蛋白酶MMP-7和MMP-13

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Keratoacanthomas are rapidly growing hyperproliferative skin tumors that may clinically or histologically be difficult to distinguish from well-differentiated squamous cell cancers (SCCs). UV light, trauma, and immune suppression represent their etiological factors. As matrix metalloproteinases (MMPs) are implicated at all stages of tumorigenesis, we investigated the expression profile of several cancer-related MMPs to find markers that would differentiate keratoacanthomas from SCCs and shed light to the pathobiology of keratoacanthoma. Samples from 31 keratoacanthomas and 15 grade I SCCs were studied using immunohistochemistry for MMP-2, -7, -8, -9, -10, -13, and -19 and p16 and laminin-52 chain. In situ hybridization for MMP-7, -10, and -13 was performed in a subset of tumors. Keratinocytes with atypia, presence of neovascularization, and composition of the inflammatory infiltrate were graded from hematoxylin–eosin stainings. MMP-7 was present in the epithelium of 4/31 keratoacanthomas and 9/15 SCCs, MMP-8 in 3/30 keratoacanthomas and 0/15 SCCs, but MMP-13 in 16/31 keratoacanthomas and 10/15 SCCs, and MMP-10 in 28/31 keratoacanthomas and all cancers. MMP-9 was detected in the epithelium in 5/31 keratoacanthomas and 8/15 SCCs, whereas MMP-2 was only present in fibroblasts in both tumors. MMP-19 was upregulated in proliferating epithelium of keratoacanthomas as was p16. Cytoplasmic laminin-52 was particularly abundant in keratinocytes at the pushing border of MMP-13-positive keratoacanthomas. We conclude that although some MMPs (MMP-10 and -13) are abundantly expressed in keratoacanthomas, the presence of MMP-7 and -9 in their epithelial pushing border is rare and should raise suspicion of SCC. Further, the loss of MMP-19 and p16 could aid in making the differential diagnosis between well-differentiated SCC and keratoacanthoma. Frequent expression of the transformation-specific MMP-13 in keratoacanthomas suggests that they are not benign tumors but incomplete SCCs.
机译：角棘皮瘤是快速增长的过度增生性皮肤肿瘤，在临床或组织学上可能难以与分化良好的鳞状细胞癌（SCC）区分开。紫外线，创伤和免疫抑制是其病因。由于基质金属蛋白酶（MMPs）在肿瘤发生的所有阶段都涉及，我们研究了几种与癌症相关的MMPs的表达谱，以寻找可将角膜棘皮瘤与SCC区别开来的标志物，并为角膜棘皮瘤的病理生物学提供了线索。使用MMP-2，-7，-8，-9，-10，-13和-19以及p16和层粘连蛋白52链的免疫组织化学研究了来自31个角膜棘皮瘤和15个I级SCC的样品。在一部分肿瘤中进行了MMP-7，-10和-13的原位杂交。具有非典型性，新血管形成和炎症浸润成分的角质形成细胞根据苏木精-伊红染色进行分级。 MMP-7存在于4/31角膜棘突和9/15 SCC的上皮中，MMP-8存在于3/30角膜棘突和0/15的SCC中，但是MMP-13存在于16/31角膜棘突和10/15的SCC中，以及MMP在28/31角棘皮瘤和所有癌症中为-10。在5/31个角棘皮瘤和8/15个鳞状细胞癌的上皮中检测到MMP-9，而MMP-2仅在两种肿瘤的成纤维细胞中都存在。 MMP-19在增殖性角棘皮瘤上皮细胞中的表达与p16一样。细胞质层粘连蛋白52在MMP-13阳性角质层棘突的推进边界处的角质形成细胞中特别丰富。我们得出的结论是，尽管一些MMP（MMP-10和-13）在角棘层瘤中大量表达，但在其上皮推动边界中MMP-7和-9的存在很少，并且应该引起对SCC的怀疑。此外，MMP-19和p16的丢失可能有助于在分化良好的SCC和角膜棘皮瘤之间进行鉴别诊断。转化特异性MMP-13在角棘层瘤中的频繁表达表明它们不是良性肿瘤，而是不完整的SCC。

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